GSBS Student Publications

Title

Aberrant termination triggers nonsense-mediated mRNA decay

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Molecular Genetics and Microbiology

Date

10-26-2005

Document Type

Article

Medical Subject Headings

*3' Untranslated Regions; *Codon, Nonsense; Poly(A)-Binding Protein I; Prions; *Protein Biosynthesis; RNA, Messenger; Saccharomyces cerevisiae Proteins

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

NMD (nonsense-mediated mRNA decay) is a cellular quality-control mechanism in which an otherwise stable mRNA is destabilized by the presence of a premature termination codon. We have defined the set of endogenous NMD substrates, demonstrated that they are available for NMD at every round of translation, and showed that premature termination and normal termination are not equivalent biochemical events. Premature termination is aberrant, and its NMD-stimulating defects can be reversed by the presence of tethered poly(A)-binding protein (Pab1p) or tethered eRF3 (eukaryotic release factor 3) (Sup35p). Thus NMD appears to be triggered by a ribosome's failure to terminate adjacent to a properly configured 3'-UTR (untranslated region), an event that may promote binding of the UPF/NMD factors to stimulate mRNA decapping.

Rights and Permissions

Citation: Biochem Soc Trans. 2006 Feb;34(Pt 1):39-42. Link to article on publisher's site

Related Resources

Link to article in PubMed

Journal Title

Biochemical Society transactions

PubMed ID

16246174