GSBS Student Publications

Student Author(s)

Cynthia J. Guidi

GSBS Program

Cell Biology

UMMS Affiliation

Department of Pathology; Department of Cell Biology



Document Type


Medical Subject Headings

Animals; Cell Transformation, Neoplastic; Chromosomal Proteins, Non-Histone; DNA-Binding Proteins; Embryonic and Fetal Development; *Gene Expression Regulation, Developmental; Genes, Tumor Suppressor; Mice; Mice, Knockout


Cell Biology | Life Sciences | Medicine and Health Sciences


SNF5/INI1 is a component of the ATP-dependent chromatin remodeling enzyme family SWI/SNF. Germ line mutations of INI1 have been identified in children with brain and renal rhabdoid tumors, indicating that INI1 is a tumor suppressor. Here we report that disruption of Ini1 expression in mice results in early embryonic lethality. Ini1-null embryos die between 3.5 and 5.5 days postcoitum, and Ini1-null blastocysts fail to hatch, form the trophectoderm, or expand the inner cell mass when cultured in vitro. Furthermore, we report that approximately 15% of Ini1-heterozygous mice present with tumors, mostly undifferentiated or poorly differentiated sarcomas. Tumor formation is associated with a loss of heterozygocity at the Ini1 locus, characterizing Ini1 as a tumor suppressor in mice. Thus, Ini1 is essential for embryo viability and for repression of oncogenesis in the adult organism.

Rights and Permissions

Citation: Mol Cell Biol. 2001 May;21(10):3598-603. Link to article on publisher's site

Related Resources

Link to article in PubMed

PubMed ID




To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.