Student Author(s)

Cynthia J. Guidi

GSBS Program

Cell Biology

UMMS Affiliation

Department of Pathology; Department of Cell Biology

Date

4-21-2001

Document Type

Article

Medical Subject Headings

Animals; Cell Transformation, Neoplastic; Chromosomal Proteins, Non-Histone; DNA-Binding Proteins; Embryonic and Fetal Development; *Gene Expression Regulation, Developmental; Genes, Tumor Suppressor; Mice; Mice, Knockout

Disciplines

Cell Biology | Life Sciences | Medicine and Health Sciences

Abstract

SNF5/INI1 is a component of the ATP-dependent chromatin remodeling enzyme family SWI/SNF. Germ line mutations of INI1 have been identified in children with brain and renal rhabdoid tumors, indicating that INI1 is a tumor suppressor. Here we report that disruption of Ini1 expression in mice results in early embryonic lethality. Ini1-null embryos die between 3.5 and 5.5 days postcoitum, and Ini1-null blastocysts fail to hatch, form the trophectoderm, or expand the inner cell mass when cultured in vitro. Furthermore, we report that approximately 15% of Ini1-heterozygous mice present with tumors, mostly undifferentiated or poorly differentiated sarcomas. Tumor formation is associated with a loss of heterozygocity at the Ini1 locus, characterizing Ini1 as a tumor suppressor in mice. Thus, Ini1 is essential for embryo viability and for repression of oncogenesis in the adult organism.

Rights and Permissions

Citation: Mol Cell Biol. 2001 May;21(10):3598-603. Link to article on publisher's site

Related Resources

Link to article in PubMed