GSBS Student Publications

Title

Nucleosome organization and targeting of SWI/SNF chromatin-remodeling complexes: contributions of the DNA sequence

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Cell Biology

Date

8-24-2007

Document Type

Article

Medical Subject Headings

Animals; *Base Sequence; *Chromatin Assembly and Disassembly; Chromosomal Proteins, Non-Histone; *DNA; Models, Genetic; *Nucleosomes; Osteocalcin; Promoter Regions (Genetics); Transcription Factors; Transcription, Genetic

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Chromatin organization within the nuclear compartment is a fundamental mechanism to regulate the expression of eukaryotic genes. During the last decade, a number of nuclear protein complexes with the ability to remodel chromatin and regulate gene transcription have been reported. Among these complexes is the SWI/SNF family, which alters chromatin structure in an ATP-dependent manner. A considerable effort has been made to understand the molecular mechanisms by which SWI/SNF catalyzes nucleosome remodeling. However, limited attention has been dedicated to studying the role of the DNA sequence in this remodeling process. Therefore, in this minireview, we discuss the contribution of nucleosome positioning and nucleosome excluding sequences to the targeting and activity of SWI/SNF complexes. This discussion includes results from our group using the rat osteocalcin gene promoter as a model. Based on these results, we postulate a model for chromatin remodeling and transcriptional activation of this gene in osteoblastic cells.

Rights and Permissions

Citation: Biochem Cell Biol. 2007 Aug;85(4):419-25. Link to article on publisher's site

DOI of Published Version

10.1139/o07-070

Related Resources

Link to Article in PubMed

Journal Title

Biochemistry and cell biology = Biochimie et biologie cellulaire

PubMed ID

17713577