Title
Translational control of the embryonic cell cycle
GSBS Program
Biochemistry & Molecular Pharmacology
UMMS Affiliation
Graduate School of Biomedical Sciences; Program in Molecular Medicine
Date
6-28-2002
Document Type
Article
Medical Subject Headings
Animals; Cell Cycle; Cell Cycle Proteins; Cyclin B; Cytoplasm; Embryo, Nonmammalian; Female; Gene Expression Regulation, Developmental; Mammals; Mitosis; Oocytes; Polyadenylation; Protein Biosynthesis; Protein-Serine-Threonine Kinases; RNA, Messenger; RNA-Binding Proteins; Transcription Factors; Tumor Cells, Cultured; *Xenopus Proteins; Xenopus laevis; *mRNA Cleavage and Polyadenylation Factors
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
The synthesis and destruction of cyclin B drives mitosis in eukaryotic cells. Cell cycle progression is also regulated at the level of cyclin B translation. In cycling extracts from Xenopus embryos, progression into M phase requires the polyadenylation-induced translation of cyclin B1 mRNA. Polyadenylation is mediated by the phosphorylation of CPEB by Aurora, a kinase whose activity oscillates with the cell cycle. Exit from M phase seems to require deadenylation and subsequent translational silencing of cyclin B1 mRNA by Maskin, a CPEB and eIF4E binding factor, whose expression is cell cycle regulated. These observations suggest that regulated cyclin B1 mRNA translation is essential for the embryonic cell cycle. Mammalian cells also display a cell cycle-dependent cytoplasmic polyadenylation, suggesting that translational control by polyadenylation might be a general feature of mitosis in animal cells.
Rights and Permissions
Citation: Cell. 2002 May 17;109(4):473-83.