GSBS Student Publications

Title

The effects of mutations in the ant promoter of phage P22 depend on context

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Molecular Genetics and Microbiology

Date

10-1-1988

Document Type

Article

Medical Subject Headings

Base Sequence; Cloning, Molecular; *Genes, Viral; *Mutation; Plasmids; *Promoter Regions (Genetics); Recombination, Genetic; Salmonella Phages; beta-Galactosidase

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Recombination was used to construct 22 two- or three-way combinations of down- and up-mutations in Pant, a strong, near-consensus promoter of phage P22. The relative strengths of these promoters in vivo were assayed by fusing them to an ant/lacZ gene fusion and measuring beta-galactosidase levels produced by lysogens carrying the fusions on single-copy prophages. The results of these assays show that the magnitude of the effect of a promoter mutation can vary considerably when its context is changed by the presence of another mutation. In addition, as Pant approaches conformity with the consensus promoter sequence, the up-mutations decrease promoter strength, even though the same mutations increase promoter strength in the presence of a down-mutation. These context effects imply that individual consensus base pairs cannot be considered to contribute to promoter strength independently.

Rights and Permissions

Citation: Genetics. 1988 Oct;120(2):319-27.

Related Resources

Link to article in PubMed

Journal Title

Genetics

PubMed ID

3143618