Differences in sequences encoding the carboxyl-terminal domain of the epidermal growth factor receptor correlate with differences in the disease potential of viral erbB genes
Biochemistry & Molecular Pharmacology
Graduate School of Biomedical Sciences; Department of Biochemistry and Molecular Biology
Medical Subject Headings
Alpharetrovirus; Animals; Avian Leukosis; Avian leukosis virus; Base Sequence; Cell Line; Cell Transformation, Neoplastic; Chick Embryo; Chickens; Epidermal Growth Factor; *Genes; *Genes, Viral; Leukemia, Erythroblastic, Acute; Receptor, Epidermal Growth Factor; Receptors, Cell Surface; Transduction, Genetic
Life Sciences | Medicine and Health Sciences
Eleven recently isolated erbB-transducing viruses as well as avian erythroblastosis virus (AEV)-R (ES4) and AEV-H have been characterized for the type of disease they cause, their ability to transform fibroblasts in culture, their ability to cause disease in pedigrees of chicken that differ in susceptibility to erbB-induced erythroblastosis, and the structure of their erbB genes. Differences in each of the biological parameters correlated with differences in erbB sequences encoding the C-terminal domain of the epidermal growth factor receptor (EGFR). Seven viruses were strain restricted in their ability to induce erythroblastosis and did not transform fibroblasts. These seven viruses contained v-erbB genes encoding the complete C terminus of the EGFR. AEV-R and AEV-H were not pedigree restricted in their ability to induce erythroblastosis and could transform fibroblasts. These viruses contain v-erbB genes that lack codons for the immediate C terminus of the EGFR. Three viruses caused angiosarcoma and one caused fibrosarcoma. The angiosarcoma and fibrosarcoma-inducing viruses were not strain restricted and did not cause erythroblastosis. The v-erbB genes of each of these viruses contained extensive internal deletions or 3' truncations in sequences encoding the C-terminal domain of the EGFR.
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Citation: Proc Natl Acad Sci U S A. 1986 Aug;83(16):6053-7.
Proceedings of the National Academy of Sciences of the United States of America
Gamett, Daniel C.; Tracy, Sharon Elizabeth; and Robinson, Harriet L., "Differences in sequences encoding the carboxyl-terminal domain of the epidermal growth factor receptor correlate with differences in the disease potential of viral erbB genes" (1986). GSBS Student Publications. 370.