Chromatin remodeling activities act on UV-damaged nucleosomes and modulate DNA damage accessibility to photolyase
Biochemistry & Molecular Pharmacology
Graduate School of Biomedical Sciences; Program in Molecular Medicine
Medical Subject Headings
Adenosine Triphosphatases; Adenosine Triphosphate; Chromatin; DNA; *DNA Damage; DNA Repair; DNA Restriction Enzymes; Deoxyribodipyrimidine Photo-Lyase; Deoxyribonuclease I; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Fungal Proteins; Nucleosomes; Time Factors; Transcription Factors; *Ultraviolet Rays
Life Sciences | Medicine and Health Sciences
Nucleosomes inhibit DNA repair in vitro, suggesting that chromatin remodeling activities might be required for efficient repair in vivo. To investigate how structural and dynamic properties of nucleosomes affect damage recognition and processing, we investigated repair of UV lesions by photolyase on a nucleosome positioned at one end of a 226-bp-long DNA fragment. Repair was slow in the nucleosome but efficient outside. No disruption or movement of the nucleosome was observed after UV irradiation and during repair. However, incubation with the nucleosome remodeling complex SWI/SNF and ATP altered the conformation of nucleosomal DNA as judged by UV photo-footprinting and promoted more homogeneous repair. Incubation with yISW2 and ATP moved the nucleosome to a more central position, thereby altering the repair pattern. This is the first demonstration that two different chromatin remodeling complexes can act on UV-damaged nucleosomes and modulate repair. Similar activities might relieve the inhibitory effect of nucleosomes on DNA repair processes in living cells.
Rights and Permissions
Citation: J Biol Chem. 2003 May 16;278(20):17655-63. Epub 2003 Mar 11. Link to article on publisher's site
DOI of Published Version
The Journal of biological chemistry
Gaillard, Helene; Fitzgerald, Daniel J.; Smith, Corey Lewis; Peterson, Craig L.; Richmond, Timothy J.; and Thoma, Fritz, "Chromatin remodeling activities act on UV-damaged nucleosomes and modulate DNA damage accessibility to photolyase" (2003). GSBS Student Publications. 365.