Title

Cytokine gene expression and protein production in peripheral blood mononuclear cells of children with acute dengue virus infections

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Graduate School of Biomedical Sciences; Center for Infectious Disease and Vaccine Research

Date

3-29-2002

Document Type

Article

Medical Subject Headings

Acute Disease; Adolescent; Child; Child, Preschool; Cytokines; Dengue Hemorrhagic Fever; Female; Fever; *Gene Expression; Humans; Infant; Interleukin-2; Interleukin-4; Leukocytes, Mononuclear; Lymphotoxin-alpha; Male; Tumor Necrosis Factor-alpha

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Plasma leakage in dengue hemorrhagic fever (DHF) is associated with elevated plasma levels of cytokines. To define further the contribution of immune activation to DHF and the source of cytokines, we analyzed the production of cytokines in peripheral blood mononuclear cells (PBMC) obtained from children with dengue, using RT-PCR and immunostaining. Tumor necrosis factor-alpha (TNF-alpha) and TNF-beta expression was detected in all samples by PCR and in < 50% of samples by immunostaining. Interferon-gamma (IFN-gamma) expression was detected in < 50% of samples by either method. Interleukin-2 (IL-2) and IL-4 expression was detected in a few samples by immunostaining but was not detectable by PCR. We found greater expression of TNF-alpha and IL-4 in DHF than in dengue fever or other (non-dengue) febrile illnesses. These results support the model of immunopathogenesis of DHF. However, low levels of cytokine expression in PBMC suggest that cellular activation in tissues may contribute to high serum cytokine levels in DHF.

Rights and Permissions

Citation: J Med Virol. 2002 May;67(1):41-6.

Related Resources

Link to article in PubMed

PubMed ID

11920816