GSBS Student Publications

Title

KCNE3 truncation mutants reveal a bipartite modulation of KCNQ1 K+ channels

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Biochemistry and Molecular Pharmacology

Date

12-2-2004

Document Type

Article

Medical Subject Headings

Amino Acid Substitution; Animals; Cells, Cultured; Ion Channel Gating; KCNQ Potassium Channels; KCNQ1 Potassium Channel; Membrane Potentials; Mutagenesis, Site-Directed; Oocytes; Potassium; Potassium Channels, Voltage-Gated; Recombinant Proteins; Structure-Activity Relationship; Xenopus laevis

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

The five KCNE genes encode a family of type I transmembrane peptides that assemble with KCNQ1 and other voltage-gated K(+) channels, resulting in potassium conducting complexes with varied channel-gating properties. It has been recently proposed that a triplet of amino acids within the transmembrane domain of KCNE1 and KCNE3 confers modulation specificity to the peptide, since swapping of these three residues essentially converts the recipient KCNE into the donor (Melman, Y.F., A. Domenech, S. de la Luna, and T.V. McDonald. 2001. J. Biol. Chem. 276:6439-6444). However, these results are in stark contrast with earlier KCNE1 deletion studies, which demonstrated that a COOH-terminal region, highly conserved between KCNE1 and KCNE3, was responsible for KCNE1 modulation of KCNQ1 (Tapper, A.R., and A.L. George. 2000 J. Gen. Physiol. 116:379-389.). To ascertain whether KCNE3 peptides behave similarly to KCNE1, we examined a panel of NH(2)- and COOH-terminal KCNE3 truncation mutants to directly determine the regions required for assembly with and modulation of KCNQ1 channels. Truncations lacking the majority of their NH(2) terminus, COOH terminus, or mutants harboring both truncations gave rise to KCNQ1 channel complexes with basal activation, a hallmark of KCNE3 modulation. These results demonstrate that the KCNE3 transmembrane domain is sufficient for assembly with and modulation of KCNQ1 channels and suggests a bipartite model for KCNQ1 modulation by KCNE1 and KCNE3 subunits. In this model, the KCNE3 transmembrane domain is active in modulation and overrides the COOH terminus' contribution, whereas the KCNE1 transmembrane domain is passive and reveals COOH-terminal modulation of KCNQ1 channels. We furthermore test the validity of this model by using the active KCNE3 transmembrane domain to functionally rescue a nonconducting, yet assembly and trafficking competent, long QT mutation located in the conserved COOH-terminal region of KCNE1.

Rights and Permissions

Citation: J Gen Physiol. 2004 Dec;124(6):759-71. Link to article on publisher's site

DOI of Published Version

10.1085/jgp.200409114

Related Resources

Link to article in PubMed

Journal Title

The Journal of general physiology

PubMed ID

15572349