Microscopic methods for measuring the elasticity of gel substrates for cell culture: microspheres, microindenters, and atomic force microscopy
Biochemistry & Molecular Pharmacology
Graduate School of Biomedical Sciences; Department of Physiology
Medical Subject Headings
Cell Culture Techniques; Elasticity; Gels; Gravitation; Microscopy, Atomic Force; *Microspheres
Life Sciences | Medicine and Health Sciences
In conjunction with surface chemistry, the mechanical properties of cell culture substrates provide important biological cues that affect cell behavior including growth, differentiation, spreading, and migration. The phenomenon has led to the increased use of biological and synthetic polymer-based flexible substrates in cell culture studies. However, widely used methods for measuring the Young's modulus have proven difficult in the characterization of these materials, as they tend to be relatively thin, soft, hydrated, and tethered to glass substrates. Here we describe three methods that have been applied successfully to probe the flexibility of soft culture substrates.
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Citation: Methods Cell Biol. 2007;83:47-65. Link to article on publisher's site
Frey, Margo Tilley; Engler, Adam; Discher, Dennis E.; Lee, Juliet; and Wang, Yu-Li, "Microscopic methods for measuring the elasticity of gel substrates for cell culture: microspheres, microindenters, and atomic force microscopy" (2007). GSBS Student Publications. Paper 359.