Augmentation of human influenza A virus-specific cytotoxic T lymphocyte memory by influenza vaccine and adjuvanted carriers (ISCOMS)
Immunology & Virology Program
Center for Infectious Disease and Vaccine Research
Medical Subject Headings
Adolescent; Adult; Double-Blind Method; Humans; ISCOMs; *Immunologic Memory; Influenza A virus; Influenza Vaccines; Middle Aged; T-Lymphocytes, Cytotoxic; Vaccination
Immunology and Infectious Disease | Life Sciences | Medicine and Health Sciences
There is a need to improve the ability of subunit vaccines to induce CD8(+) CTL responses in humans, especially for vaccines used to prevent illness by organisms that undergo antigenic variation at their major neutralizing antibody sites, e.g., influenza A viruses and human immunodeficiency virus. Murine models have demonstrated the protective role of cross-reactive CTL against influenza A virus antigenic drift. We tested the ability of an adjuvanted carrier (Iscomatrix) to help human antigen-presenting cells present formalin-killed influenza vaccine to human CD8(+) CTL clones in vitro and in vaccinated humans. The results of a randomized, double-blind, controlled clinical study demonstrate that a single dose of a vaccine formulated into Iscom particles increased influenza A virus-specific CTL memory in 50-60% of recipients, compared to 5% of the recipients of the standard influenza vaccine.
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Citation: Virology. 1999 Jul 5;259(2):256-61. Link to article on publisher's site
Ennis, Francis A.; Cruz, John; Jameson, Julie Marie; Klein, Michael D.; Burt, David; and Thipphawong, John, "Augmentation of human influenza A virus-specific cytotoxic T lymphocyte memory by influenza vaccine and adjuvanted carriers (ISCOMS)" (1999). GSBS Student Publications. 342.