Title

Functional proteomics reveals the biochemical niche of C. elegans DCR-1 in multiple small-RNA-mediated pathways

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Graduate School of Biomedical Sciences; Program in Molecular Medicine

Date

1-28-2006

Document Type

Article

Medical Subject Headings

Amino Acid Sequence; Animals; Binding, Competitive; Caenorhabditis elegans; Caenorhabditis elegans Proteins; DNA-Binding Proteins; Endoribonucleases; Exoribonucleases; Gene Deletion; Mass Spectrometry; MicroRNAs; Models, Biological; Molecular Sequence Data; Molecular Structure; Proteomics; RNA Interference; RNA Replicase; RNA, Small Interfering; Sequence Alignment; Signal Transduction

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

In plants, animals, and fungi, members of the Dicer family of RNase III-related enzymes process double-stranded RNA (dsRNA) to initiate small-RNA-mediated gene-silencing mechanisms. To learn how C. elegans Dicer, DCR-1, functions in multiple distinct silencing mechanisms, we used a mass-spectrometry-based proteomics approach to identify DCR-1-interacting proteins. We then generated and characterized deletion alleles for the corresponding genes. The interactors are required for production of three species of small RNA, including (1) small interfering RNAs (siRNAs), derived from exogenous dsRNA triggers (exo-siRNAs); (2) siRNAs derived from endogenous triggers (endo-siRNAs); and (3) developmental regulatory microRNAs (miRNAs). One interactor, the conserved RNA-phosphatase homolog PIR-1, is required for the processing of a putative amplified DCR-1 substrate. Interactors required for endo-siRNA production include ERI-1 and RRF-3, whose loss of function enhances RNAi. Our findings provide a first glimpse at the complex biochemical niche of Dicer and suggest that competition exists between DCR-1-mediated small-RNA pathways.

Rights and Permissions

Citation: Cell. 2006 Jan 27;124(2):343-54. Link to article on publisher's site

Related Resources

Link to article in PubMed