GSBS Student Publications

Title

Functional association of class II antigens with cell surface binding of Epstein-Barr virus

GSBS Program

Biochemistry & Molecular Pharmacology

Date

6-1-1985

Document Type

Article

Medical Subject Headings

Animals; Antibodies, Neoplasm; Antigens, Surface; Antilymphocyte Serum; Binding, Competitive; Burkitt Lymphoma; Cell Line; Electrophoresis, Polyacrylamide Gel; HLA-DR Antigens; Herpesvirus 4, Human; Histocompatibility Antigens Class II; Humans; Membrane Proteins; Neoplasm Proteins; Rabbits; Receptors, Complement 3d; Receptors, Virus

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

A functional role of class II antigen in the binding of Epstein-Barr virus (EBV) was deduced from the study of membrane proteins on Jijoye, an EBV receptor (EBVR)-positive B cell line, and its mutant, EBVR-negative daughter cell line, P3HR-1. From gel electrophoresis of radiolabeled microsomal membrane proteins and immunoprecipitates, we identified class II antigen on Jijoye but not on P3HR-1 cells and the presence of Ii on both cell lines. The role of these molecules in EBVR function was tested by antibody blocking of virus adsorption. Anti-p23,30 serum (to class II antigen) was found to block binding of EBV to B lymphoblasts under conditions in which normal rabbit serum, rabbit antiserum to butyrate-treated P3HR-1 cells (with ample anti-Ii antibodies), and rabbit anti-p44,12 (to class I antigen and beta 2-microglobulin) serum did not block virus binding. Only one of four commercial monoclonal antibodies (MoAb) to framework epitopes on class II antigens blocked binding of EBV, whereas all four MoAb demonstrated immunofluorescent reactivity with the EBVR+ Raji cells. In previous studies of binding of EBV to hairy leukemic cells, a substantial subpopulation of HLA-DR+, EBVR- cells was identified, in addition to HLA-DR+, EBVR+ cells. These findings were consistent with the view that the HLA-DR complex has a role in the binding of EBV but that other components are also needed for the expression of EBVR function.

Rights and Permissions

Citation: J Immunol. 1985 Jun;134(6):3776-80.

Related Resources

Link to Article in PubMed

Journal Title

Journal of immunology (Baltimore, Md. : 1950)

PubMed ID

2985696