GSBS Student Publications

Title

Kinetics of hairpin ribozyme cleavage in yeast

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Biochemistry and Molecular Biology

Date

9-18-1997

Document Type

Article

Medical Subject Headings

Antisense Elements (Genetics); Base Sequence; Kinetics; Molecular Sequence Data; Mutation; Nucleic Acid Conformation; Phosphoglycerate Kinase; Poly A; RNA Caps; RNA, Catalytic; RNA, Messenger; Yeasts

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Hairpin ribozymes catalyze a self-cleavage reaction that provides a simple model for quantitative analyses of intracellular mechanisms of RNA catalysis. Decay rates of chimeric mRNAs containing self-cleaving ribozymes give a direct measure of intracellular cleavage kinetics in yeast. Intracellular ribozyme-mediated cleavage occurs at similar rates and shows similar inhibition by ribozyme mutations as ribozyme-mediated reactions in vitro, but only when ribozymes are located in a favorable mRNA sequence context. The impact of cleavage on mRNA abundance is shown to depend directly on intrinsic mRNA stability. Surprisingly, cleavage products are no more labile than uncleaved mRNAs despite the loss of terminal cap structures or poly (A).

Rights and Permissions

Citation: RNA. 1997 Sep;3(9):961-73.

Related Resources

Link to article in PubMed

Journal Title

RNA (New York, N.Y.)

PubMed ID

9292496