GSBS Student Publications

Student Author(s)

Serene Forte

GSBS Program

Immunology & Virology Program

UMMS Affiliation

Department of Pediatrics

Date

2-5-2000

Document Type

Article

Medical Subject Headings

Amino Acid Sequence; CD4-Positive T-Lymphocytes; Child; Child, Preschool; Cloning, Molecular; Cytopathogenic Effect, Viral; HIV-1; Humans; Jurkat Cells; Kinetics; Molecular Sequence Data; Phenotype; Polymerase Chain Reaction; Receptors, CCR5; Sequence Alignment; Viral Envelope Proteins; Virus Replication

Disciplines

Life Sciences | Medicine and Health Sciences | Virus Diseases

Abstract

We analyzed the env genes of cytopathic and noncytopathic biological clones derived from two HIV-1-infected children with discordant clinical courses. Chimeric viruses were constructed by switching env regions from V2 through V3 of the biological clones with the corresponding region from the molecular clone NL4-3. These HIV-1 chimeric viruses exhibited similar replication kinetics as well as syncytium-inducing abilities. The chimeric virus containing the env region of noncytopathic biological clone, GC6 8-4, was noncytopathic in an in vitro cell-killing assay, while the chimeric virus containing the env region of cytopathic biological clone, HC4, was cytopathic in the in vitro cell-killing assay. These studies suggest the presence of a cytopathicity determinant that maps to the envelope sequences contained within the downstream region of V2 and within the V3 region (nucleotide position 6822 to nucleotide position 7250, based on NL4-3 sequence).

Rights and Permissions

Citation: AIDS Res Hum Retroviruses. 2000 Jan 20;16(2):125-37. Link to article on publisher's site

Comments

This is a copy of an article published in AIDS Research and Human Retroviruses ©2000 Mary Ann Liebert, Inc.; AIDS Research and Human Retroviruses is available online at: http://www.liebertonline.com.

Related Resources

Link to article in PubMed

Journal Title

AIDS research and human retroviruses

PubMed ID

10659052

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