GSBS Program
Immunology & Virology Program
UMMS Affiliation
Department of Pediatrics
Date
2-5-2000
Document Type
Article
Medical Subject Headings
Amino Acid Sequence; CD4-Positive T-Lymphocytes; Child; Child, Preschool; Cloning, Molecular; Cytopathogenic Effect, Viral; HIV-1; Humans; Jurkat Cells; Kinetics; Molecular Sequence Data; Phenotype; Polymerase Chain Reaction; Receptors, CCR5; Sequence Alignment; Viral Envelope Proteins; Virus Replication
Disciplines
Life Sciences | Medicine and Health Sciences | Virus Diseases
Abstract
We analyzed the env genes of cytopathic and noncytopathic biological clones derived from two HIV-1-infected children with discordant clinical courses. Chimeric viruses were constructed by switching env regions from V2 through V3 of the biological clones with the corresponding region from the molecular clone NL4-3. These HIV-1 chimeric viruses exhibited similar replication kinetics as well as syncytium-inducing abilities. The chimeric virus containing the env region of noncytopathic biological clone, GC6 8-4, was noncytopathic in an in vitro cell-killing assay, while the chimeric virus containing the env region of cytopathic biological clone, HC4, was cytopathic in the in vitro cell-killing assay. These studies suggest the presence of a cytopathicity determinant that maps to the envelope sequences contained within the downstream region of V2 and within the V3 region (nucleotide position 6822 to nucleotide position 7250, based on NL4-3 sequence).
Rights and Permissions
Citation: AIDS Res Hum Retroviruses. 2000 Jan 20;16(2):125-37. Link to article on publisher's site
Comments
This is a copy of an article published in AIDS Research and Human Retroviruses ©2000 Mary Ann Liebert, Inc.; AIDS Research and Human Retroviruses is available online at: http://www.liebertonline.com.