GSBS Program
Immunology & Virology Program
UMMS Affiliation
Department of Pediatrics; Program in Molecular Medicine
Date
12-1-1994
Document Type
Article
Medical Subject Headings
Acquired Immunodeficiency Syndrome; CD4 Lymphocyte Count; Child, Preschool; Genes, gag; Giant Cells; HIV Core Protein p24; HIV-1; Humans; Infant; Phenotype; RNA, Viral; T-Lymphocytes; Tumor Cells, Cultured; *Virus Replication
Disciplines
Life Sciences | Medicine and Health Sciences | Virus Diseases
Abstract
Human immunodeficiency virus type 1 (HIV-1) isolates from six infected individuals less then 4 years of age were phenotyped for their syncytium-inducing (SI) ability in MT-2 cells. Three viral isolates that induced syncytia were detected. One SI isolate was from an individual who was in disease stage P2A,B,C and two SI isolates were recovered sequentially from another individual who switched from disease stage P1B to P2F. Non-syncytium-inducing (NSI) isolates were detected in two individuals who were in stage P1B of disease, and in a third individual who was in stage P2A of disease. Three sequential isolates obtained over a 2-year period from a fourth individual who progressed from disease stage P1B to P2A,B,C and subsequently died of AIDS-related disease were also found to have the NSI phenotype. To test whether NSI isolates can replicate in the absence of syncytium formation, we analyzed NSI-infected MT-2 cells for production of viral p24 antigen and expression of viral RNA by in situ hybridization. By day 12 postinfection, 6 of 7 NSI viral isolates produced 7- to 36-fold increases in p24 antigen compared to day 6, and expressed viral RNA in 13-20% of cells. A single NSI isolate that did not replicate in MT-2 cells was obtained from an individual who was asymptomatic (stage P1B). The individual rapidly progressed to symptomatic stage P2F and two sequential SI viruses were isolated. These SI isolates replicated in MT-2 cells and induced cytopathic effects.(ABSTRACT TRUNCATED AT 250 WORDS)
Rights and Permissions
Citation: AIDS Res Hum Retroviruses. 1994 Dec;10(12):1613-8. doi:10.1089/aid.1994.10.1613. Link to article on publisher's website
Comments
This is a copy of an article published in AIDS Research and Human Retroviruses © 1994 Mary Ann Liebert, Inc.; AIDS Research and Human Retroviruses is available online at: http://www.liebertonline.com.