GSBS Student Publications

Student Author(s)

Serene Forte

UMMS Affiliation

Department of Pediatrics; Program in Molecular Medicine

Date

12-1-1994

Document Type

Article

Medical Subject Headings

Acquired Immunodeficiency Syndrome; CD4 Lymphocyte Count; Child, Preschool; Genes, gag; Giant Cells; HIV Core Protein p24; HIV-1; Humans; Infant; Phenotype; RNA, Viral; T-Lymphocytes; Tumor Cells, Cultured; *Virus Replication

Disciplines

Life Sciences | Medicine and Health Sciences | Virus Diseases

Abstract

Human immunodeficiency virus type 1 (HIV-1) isolates from six infected individuals less then 4 years of age were phenotyped for their syncytium-inducing (SI) ability in MT-2 cells. Three viral isolates that induced syncytia were detected. One SI isolate was from an individual who was in disease stage P2A,B,C and two SI isolates were recovered sequentially from another individual who switched from disease stage P1B to P2F. Non-syncytium-inducing (NSI) isolates were detected in two individuals who were in stage P1B of disease, and in a third individual who was in stage P2A of disease. Three sequential isolates obtained over a 2-year period from a fourth individual who progressed from disease stage P1B to P2A,B,C and subsequently died of AIDS-related disease were also found to have the NSI phenotype. To test whether NSI isolates can replicate in the absence of syncytium formation, we analyzed NSI-infected MT-2 cells for production of viral p24 antigen and expression of viral RNA by in situ hybridization. By day 12 postinfection, 6 of 7 NSI viral isolates produced 7- to 36-fold increases in p24 antigen compared to day 6, and expressed viral RNA in 13-20% of cells. A single NSI isolate that did not replicate in MT-2 cells was obtained from an individual who was asymptomatic (stage P1B). The individual rapidly progressed to symptomatic stage P2F and two sequential SI viruses were isolated. These SI isolates replicated in MT-2 cells and induced cytopathic effects.(ABSTRACT TRUNCATED AT 250 WORDS)

Rights and Permissions

Citation: AIDS Res Hum Retroviruses. 1994 Dec;10(12):1613-8. doi:10.1089/aid.1994.10.1613. Link to article on publisher's website

Comments

This is a copy of an article published in AIDS Research and Human Retroviruses © 1994 Mary Ann Liebert, Inc.; AIDS Research and Human Retroviruses is available online at: http://www.liebertonline.com.

Related Resources

Link to article in PubMed

Journal Title

AIDS research and human retroviruses

PubMed ID

7888220

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.