Effect of CpG methylation on isotype and magnitude of antibody responses to influenza hemagglutinin-expressing plasmid
Biochemistry & Molecular Pharmacology
Graduate School of Biomedical Sciences; Department of Pathology
Medical Subject Headings
Animals; Antibodies, Viral; Cells, Cultured; *CpG Islands; DNA; *DNA Methylation; Female; Hemagglutinin Glycoproteins, Influenza Virus; Lipopolysaccharides; Mice; Mice, Inbred BALB C; *Plasmids; Promoter Regions (Genetics); Th1 Cells
Life Sciences | Medicine and Health Sciences
We previously showed that intramuscular saline DNA immunizations favor the development of an IgG2a-dominant Th1 immune response, whereas gene gun DNA immunizations stimulate the production of an IgG1-dominant Th2 immune response. Several studies have implicated immunostimulatory CpG sequences as the causative factor in the development of Th1 immune responses to saline DNA immunization. To determine whether the Th1 cytokine-inducing properties of CpG sequences in plasmid DNA (pDNA) were responsible for the induction of a Th1 immune response, in vitro methylated and untreated (nonmethylated) hemagglutinin-expressing pDNA were compared for immunogenicity. Methylation abrogated the immunostimulatory activity of pDNA for cultured splenocytes and significantly reduced antigen expression. However, methylation of pDNA was not associated with a change from the induction of IgG2a to IgG1. After immunization with the methylated plasmid, the magnitude of the immune response was reduced. However, the decline in the total antibody response matched the decline in antigen expression. The dose of DNA or the presence of lipopolysaccharide in pDNA likewise did not affect the preferential development of an IgG2a antibody response. Our findings reveal that high levels of CpG sequences are not required for raising IgG2a-predominant, Thl-biased immune responses to intramuscular injections of hemagglutinin-expressing DNA.
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Citation: DNA Cell Biol. 1999 Sep;18(9):663-70. Link to article on publisher's site
DNA and cell biology
Feltquate, David Marc and Robinson, Harriet L., "Effect of CpG methylation on isotype and magnitude of antibody responses to influenza hemagglutinin-expressing plasmid" (1999). GSBS Student Publications. 286.