GSBS Student Publications

Title

Localization of motor-related proteins and associated complexes to active, but not inactive, centromeres

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Cell Biology

Date

5-16-1998

Document Type

Article

Medical Subject Headings

Biological Markers; Centromere; Chromosomal Proteins, Non-Histone; Dynein ATPase; Fluorescent Antibody Technique, Indirect; Humans; Kinesin; Microfilament Proteins; Microtubule Proteins; Microtubule-Associated Proteins; Tumor Cells, Cultured

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

Multicentric chromosomes are often found in tumor cells and certain cell lines. How they are generated is not fully understood, though their stability suggests that they are non-functional during chromosome segregation. Growing evidence has implicated microtubule motor proteins in attachment of chromosomes to the mitotic spindle and in chromosome movement. To better understand the molecular basis for the inactivity of centromeres associated with secondary constrictions, we have tested these structures by immunofluorescence microscopy for the presence of motor complexes and associated proteins. We find strong immunoreactivity at the active, but not inactive, centromeres of prometaphase multicentric chromosomes using antibodies to the cytoplasmic dynein intermediate chains, three components of the dynactin complex (dynamitin, Arp1 and p150 Glued ), the kinesin-related proteins CENP-E and MCAK and the proposed structural and checkpoint proteins HZW10, CENP-F and Mad2p. These results offer new insight into the assembly and composition of both primary and secondary constrictions and provide a molecular basis for the apparent inactivity of the latter during chromosome segregation.

Rights and Permissions

Citation: Hum Mol Genet. 1998 Apr;7(4):671-7.

Related Resources

Link to article in PubMed

Journal Title

Human molecular genetics

PubMed ID

9499420