GSBS Student Publications

Title

Internal synthesis of p23,30 by several lymphoid malignancies

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Medicine, Division of Hematology/Oncology; Department of Pathology

Date

2-1-1979

Document Type

Article

Medical Subject Headings

Antigens, Surface; Autoradiography; Cell Membrane; Cytotoxicity Tests, Immunologic; Electrophoresis, Polyacrylamide Gel; Epitopes; *HLA Antigens; Humans; Immune Sera; Isoantigens; Leukemia; Lymphoma; Membrane Proteins; Precipitation

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

The aim of this study was to prove the internal synthesis of p23,30 antigen (HLA-D related determinant) on human leukemias and lymphomas on which it has been detected with complement-dependent cytotoxic assays. Murine Ia antigens similar to p23,30 antigen are found on many subsets of cells in the mouse (B lymphocytes, macrophages, allogeneically activated T lymphocytes) and on intercellularly transferred immunoregulatory molecules, which may be adsorbed to other cells. The question exists whether the p23,30 antigen, which occurs on a wide range of human leukemias, is internally synthesized by these tumors or, in some instances, is synthesized by normal lymphocytes and is adsorbed to the leukemic cells. The expression of p23,30 antigen on a limited series of human leukemias and lymphomas was detected by a complement dependent, cytotoxicity assay. The internal synthesis of p23,30 antigen and p44,12 (HLA-A and -B antigens and beta2-microglobulin) was confirmed by immunoprecipitation and these antigens from [35S]methionine labeled, detergent solubilized membranes of tumor cells. In each instance, the synthesis of p23,30 antigen by the malignant cells was confirmed. The distribution of p23,30 antigen (and 1a antigen) on subsets of normal cells and in immunoregulatory molecules was reviewed. In view of these findings, the role of p23,30 antigen in the diagnosis of subsets of human hematologic malignancies was reconsidered.

Rights and Permissions

Citation: Exp Hematol. 1979 Feb;7(2):94-104.

Related Resources

Link to article in PubMed

Journal Title

Experimental hematology

PubMed ID

85554

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