GSBS Student Publications

Title

Small B cells as antigen-presenting cells in the induction of tolerance to soluble protein antigens

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Molecular Genetics and Microbiology

Date

1-1-1992

Document Type

Article

Medical Subject Headings

Animals; Antigen-Presenting Cells; B-Lymphocytes; Chickens; Female; Globulins; *Immune Tolerance; Immunoglobulin D; Immunoglobulin Fab Fragments; Immunosuppressive Agents; Immunotherapy, Adoptive; Mice; Mice, Inbred BALB C; Mice, Inbred Strains; Organic Chemicals; Rabbits

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

We have investigated the ability of resting B cells, acting as antigen-presenting cells, to induce tolerance to soluble protein antigens in mice, using an antigen targeted specifically to B cells. We inject mice intravenously with ultracentrifuged Fab fragments of rabbit anti-mouse immunoglobulin D (IgD) (Fab anti-delta). Treatment with Fab anti-delta results in profound tolerance to challenge with 100 micrograms Fab nonimmune rabbit Ig (Fab NRG), precipitated in alum, as measured by antibody production. Tolerance to rabbit Fab is antigen specific, since the treated mice make normal antibody responses to a control antigen, chicken Ig. Tolerance is dependent on antigen presentation by B cells, since intravenous injection of soluble Fab NRG, which is not targeted to B cells, results in a much lower frequency and degree of tolerance, especially at lower doses. T cell help in this system is affected, since T cells from Fab anti-delta-treated mice fail to provide help for an adoptive primary antibody response to Fab NRG when transferred together with normal B cells into severe combined immunodeficient (SCID) mice. The antigen-specific B cell compartment is also affected during tolerance induction, since B cells from treated animals make less antibody than normal B cells when transferred into SCID mice with normal T cells. Although the mechanism of nonresponsiveness in the helper T cell compartment remains to be determined, we think it is likely that the precursors of helper T cells are inactivated or deleted by encountering antigen presented by small, resting B cells, which lack accessory signals necessary to induce helper T cell proliferation and differentiation to effector function.(ABSTRACT TRUNCATED AT 250 WORDS)

Rights and Permissions

Citation: J Exp Med. 1992 Jan 1;175(1):131-8.

Related Resources

Link to article in PubMed

Journal Title

The Journal of experimental medicine

PubMed ID

1730913