GSBS Student Publications

Title

Structure, exchange determinants, and family-wide rab specificity of the tandem helical bundle and Vps9 domains of Rabex-5

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Graduate School of Biomedical Sciences; Program in Molecular Medicine and Department of Biochemistry and Molecular Pharmacology

Date

9-2-2004

Document Type

Article

Medical Subject Headings

Amino Acid Sequence; Animals; Carrier Proteins; Catalytic Domain; Endocytosis; Guanine Nucleotide Exchange Factors; Humans; *Intracellular Signaling Peptides and Proteins; Models, Molecular; Molecular Sequence Data; Mutation; Phenylalanine; Protein Binding; Protein Structure, Secondary; Sequence Homology, Amino Acid; rab GTP-Binding Proteins; rab5 GTP-Binding Proteins

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

The Rab5 GTPase, an essential regulator of endocytosis and endosome biogenesis, is activated by guanine-nucleotide exchange factors (GEFs) that contain a Vps9 domain. Here, we show that the catalytic core of the Rab GEF Rabex-5 has a tandem architecture consisting of a Vps9 domain stabilized by an indispensable helical bundle. A family-wide analysis of Rab specificity demonstrates high selectivity for Rab5 subfamily GTPases. Conserved exchange determinants map to a common surface of the Vps9 domain, which recognizes invariant aromatic residues in the switch regions of Rab GTPases and selects for the Rab5 subfamily by requiring a small nonacidic residue preceding a critical phenylalanine in the switch I region. These and other observations reveal unexpected similarity with the Arf exchange site in the Sec7 domain.

Rights and Permissions

Citation: Cell. 2004 Sep 3;118(5):607-17. Link to article on publisher's site

DOI of Published Version

10.1016/j.cell.2004.08.009

Related Resources

Link to article in PubMed

Journal Title

Cell

PubMed ID

15339665