GSBS Student Publications

Title

A lipidated anti-Tat antibody enters living cells and blocks HIV-1 viral replication

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Medicine, Division of Pulmonary, Allergy & Critical Care

Date

3-1-1997

Document Type

Article

Medical Subject Headings

Antibodies, Monoclonal; Biological Transport; Cells, Cultured; Enzyme-Linked Immunosorbent Assay; Gene Products, tat; Glycine; HIV Antibodies; HIV Core Protein p24; HIV Reverse Transcriptase; HIV-1; Immunoglobulin G; Lipoproteins; Lymphocytes; Microscopy, Fluorescence; Virus Latency; Virus Replication; tat Gene Products, Human Immunodeficiency Virus

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

We have developed a chemical modification of antibodies, lipidation, which enables their intracellular delivery into living cells. Intracellular localization of lipidated antibodies was demonstrated by confocal microscopy and by measuring cellular uptake of 125I-labeled lipidated antibodies. Functionally, a lipidated monoclonal antibody directed against the Tat protein from human immunodeficiency virus type 1 (HIV-1) inhibited viral replication of several HIV-1 isolates by approximately 85% as shown by increased viability of infected cells and decreased reverse transcriptase activity. The antibody in its native form had no such effect. These data show that lipidated antibodies can reach and functionally inhibit intracellular targets. Lipidation may help to facilitate the development of intracellular immunotherapy for AIDS.

Rights and Permissions

Citation: J Acquir Immune Defic Syndr Hum Retrovirol. 1997 Mar 1;14(3):193-203.

Related Resources

Link to article in PubMed

Journal Title

Journal of acquired immune deficiency syndromes and human retrovirology : official publication of the International Retrovirology Association

PubMed ID

9117450