GSBS Student Publications

Title

Bone metabolism in the osteopetrotic rat mutation microphthalmia blanc

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Anatomy; Department of Cell Biology

Date

5-1-1995

Document Type

Article

Medical Subject Headings

Adenosine Triphosphatases; Animals; Blotting, Northern; Bone Development; Bone Resorption; Carbonic Anhydrases; Disease Models, Animal; Female; Gene Expression Regulation, Enzymologic; Male; Mice; Mice, Mutant Strains; Microphthalmos; Microscopy, Electron; Mutation; Osteoclasts; Osteopetrosis; RNA, Messenger; Rats

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

We have examined parameters of bone metabolism in a new mutation, microphthalmia blanc (mib), in the rat exhibiting a skeletal sclerosis at birth that improves with age. There were no significant differences in the rate of bone formation during the first postnatal month except a temporary reduction in mutants at 3 weeks that coincided with compromised nutrition at weaning. At birth the ruffled border in mutant osteoclasts was absent or poorly developed and mRNA analyses of mutant bone compared to normal bone showed significant reductions in the messages for the osteoclast-specific genes carbonic andydrase II and tartrate-resistant ATPase. These distinctive ultrastructural and molecular differences were not present 1 month later. These data show that the transient osteopetrosis in mib rats results from a perinatal reduction in ultrastructural and enzymatic features of active osteoclasts and is not complicated by elevations in bone formation. The molecular basis for both the production and resolution of these abnormalities deserves further study.

Rights and Permissions

Citation: Bone. 1995 May;16(5):567-74.

Related Resources

Link to article in PubMed

Journal Title

Bone

PubMed ID

7654471