GSBS Student Publications

Title

Paralog group 1 hox genes regulate rhombomere 5/6 expression of vhnf1, a repressor of rostral hindbrain fates, in a meis-dependent manner

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Graduate School of Biomedical Sciences; Program in Neuroscience; Department of Biochemistry and Molecular Pharmacology

Date

6-30-2004

Document Type

Article

Medical Subject Headings

Animals; Body Patterning; *Epistasis, Genetic; *Gene Expression Regulation, Developmental; Gene Transfer Techniques; Genes, Homeobox; Immunohistochemistry; In Situ Hybridization; Microinjections; Morphogenesis; RNA, Messenger; Rhombencephalon; Zebrafish

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

The vertebrate hindbrain is segmented into an array of rhombomeres (r), but it remains to be fully understood how segmentation is achieved. Here we report that reducing meis function transforms the caudal hindbrain to an r4-like fate, and we exploit this experimental state to explore how r4 versus r5-r6 segments are set aside. We demonstrate that r4 transformation of the caudal hindbrain is mediated by paralog group 1 (PG1) hox genes and can be repressed by vhnf1, a gene expressed in r5-r6. We further find that vhnf1 expression is regulated by PG1 hox genes in a meis-dependent manner. This implies that PG1 hox genes not only induce r4 fates throughout the caudal hindbrain, but also induce expression of vhnf1, which then represses r4 fates in the future r5-r6. Our results further indicate that r4 transformation of the caudal hindbrain occurs at intermediate levels of meis function, while extensive removal of meis function produces a hindbrain completely devoid of segments, suggesting that different hox-dependent processes may have distinct meis requirements. Notably, reductions in the function of another Hox cofactor, pbx, have not been reported to transform the caudal hindbrain, suggesting that Meis and Pbx proteins may also function differently in their roles as Hox cofactors.

Rights and Permissions

Citation: Dev Biol. 2004 Jul 15;271(2):350-61. Link to article on publisher's site

DOI of Published Version

10.1016/j.ydbio.2004.04.007

Related Resources

Link to article in PubMed

Journal Title

Developmental biology

PubMed ID

15223339