GSBS Student Publications

Student Author(s)

Paola N. Perrat

GSBS Program

Neuroscience

UMMS Affiliation

Department of Neurobiology; Waddell Lab; Graduate School of Biomedical Sciences, Neuroscience Program

Date

3-16-2016

Document Type

Article

Disciplines

Neuroscience and Neurobiology

Abstract

Memories are stored in the fan-out fan-in neural architectures of the mammalian cerebellum and hippocampus and the insect mushroom bodies. However, whereas key plasticity occurs at glutamatergic synapses in mammals, the neurochemistry of the memory-storing mushroom body Kenyon cell output synapses is unknown. Here we demonstrate a role for acetylcholine (ACh) in Drosophila. Kenyon cells express the ACh-processing proteins ChAT and VAChT, and reducing their expression impairs learned olfactory-driven behavior. Local ACh application, or direct Kenyon cell activation, evokes activity in mushroom body output neurons (MBONs). MBON activation depends on VAChT expression in Kenyon cells and is blocked by ACh receptor antagonism. Furthermore, reducing nicotinic ACh receptor subunit expression in MBONs compromises odor-evoked activation and redirects odor-driven behavior. Lastly, peptidergic corelease enhances ACh-evoked responses in MBONs, suggesting an interaction between the fast- and slow-acting transmitters. Therefore, olfactory memories in Drosophila are likely stored as plasticity of cholinergic synapses.

Rights and Permissions

Citation: Neuron. 2016 Mar 16;89(6):1237-47. doi: 10.1016/j.neuron.2016.02.015. Epub 2016 Mar 3. Link to article on publisher's website

Comments

Open access funded by Wellcome Trust.

Related Resources

Link to article in PubMed

Journal Title

Neuron

PubMed ID

26948892

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

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