GSBS Student Publications

Title

Staufen1 senses overall transcript secondary structure to regulate translation

Student Author(s)

Erin E. Heyer

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology

Date

1-2014

Document Type

Article

Disciplines

Biochemistry | Molecular Biology | Structural Biology

Abstract

Human Staufen1 (Stau1) is a double-stranded RNA (dsRNA)-binding protein implicated in multiple post-transcriptional gene-regulatory processes. Here we combined RNA immunoprecipitation in tandem (RIPiT) with RNase footprinting, formaldehyde cross-linking, sonication-mediated RNA fragmentation and deep sequencing to map Staufen1-binding sites transcriptome wide. We find that Stau1 binds complex secondary structures containing multiple short helices, many of which are formed by inverted Alu elements in annotated 3' untranslated regions (UTRs) or in 'strongly distal' 3' UTRs. Stau1 also interacts with actively translating ribosomes and with mRNA coding sequences (CDSs) and 3' UTRs in proportion to their GC content and propensity to form internal secondary structure. On mRNAs with high CDS GC content, higher Stau1 levels lead to greater ribosome densities, thus suggesting a general role for Stau1 in modulating translation elongation through structured CDS regions. Our results also indicate that Stau1 regulates translation of transcription-regulatory proteins.

Rights and Permissions

Citation: Nat Struct Mol Biol. 2014 Jan;21(1):26-35. doi: 10.1038/nsmb.2739. Epub 2013 Dec 15. Link to article on publisher's site

Related Resources

Link to Article in PubMed

Journal Title

Nature structural and molecular biology

PubMed ID

24336223