GSBS Student Publications

Student Author(s)

Kendra Takle

GSBS Program

Neuroscience

UMMS Affiliation

Department of Neurobiology; Yang Xiang Lab; Graduate School of Biomedical Sciences, Neuroscience Program

Date

10-20-2015

Document Type

Article

Disciplines

Neuroscience and Neurobiology

Abstract

The transient receptor potential A1 (TRPA1) channel is an evolutionarily conserved detector of temperature and irritant chemicals. Here, we show that two specific isoforms of TRPA1 in Drosophila are H2O2 sensitive and that they can detect strong UV light via sensing light-induced production of H2O2. We found that ectopic expression of these H2O2-sensitive Drosophila TRPA1 (dTRPA1) isoforms conferred UV sensitivity to light-insensitive HEK293 cells and Drosophila neurons, whereas expressing the H2O2-insensitive isoform did not. Curiously, when expressed in one specific group of motor neurons in adult flies, the H2O2-sensitive dTRPA1 isoforms were as competent as the blue light-gated channelrhodopsin-2 in triggering motor output in response to light. We found that the corpus cardiacum (CC) cells, a group of neuroendocrine cells that produce the adipokinetic hormone (AKH) in the larval ring gland endogenously express these H2O2-sensitive dTRPA1 isoforms and that they are UV sensitive. Sensitivity of CC cells required dTRPA1 and H2O2 production but not conventional phototransduction molecules. Our results suggest that specific isoforms of dTRPA1 can sense UV light via photochemical production of H2O2. We speculate that UV sensitivity conferred by these isoforms in CC cells may allow young larvae to activate stress response--a function of CC cells--when they encounter strong UV, an aversive stimulus for young larvae.

Rights and Permissions

Citation: Proc Natl Acad Sci U S A. 2015 Oct 20;112(42):E5753-61. doi: 10.1073/pnas.1514862112. Epub 2015 Oct 6. Link to article on publisher's site

DOI of Published Version

10.1073/pnas.1514862112

Comments

Publisher's PDF will be posted as allowed by publisher's author rights policy at http://www.pnas.org/site/aboutpnas/rightpermfaq.xhtml.

Related Resources

Link to Article in PubMed

Keywords

Drosophila, UV sensing, dTRPA1 isoforms, optogenetics, reactive oxygen species

Journal Title

Proceedings of the National Academy of Sciences of the United States of America

PubMed ID

26443856

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