GSBS Student Publications

Title

Preparation of early Drosophila embryonic fillets (before cuticle deposition)

Student Author(s)

Preethi Ramachandran

GSBS Program

Neuroscience

UMMS Affiliation

Department of Neurobiology; Budnik Lab; Graduate School of Biomedical Sciences, Neuroscience Program

Date

10-1-2010

Document Type

Article

Medical Subject Headings

Animals; Cells, Cultured; Drosophila; Histological Techniques; Insect Proteins; Oogonia

Disciplines

Cell Biology | Developmental Biology | Laboratory and Basic Science Research | Neuroscience and Neurobiology

Abstract

This article describes the dissection of early Drosophila embryos to generate flat or fillet preparations. For the procedure, a modified chamber or well is designed using glass slides and sealant. Embryos are dissected by removing the chorionic (outer) membrane (performed outside the chamber) and then removing the vitelline (inner) membrane (performed inside the chamber and under saline).

Rights and Permissions

Citation: Cold Spring Harb Protoc. 2010 Oct 1;2010(10):pdb.prot5497. doi: 10.1101/pdb.prot5497. Link to article on publisher's site.

Related Resources

Link to Article in PubMed

Journal Title

Cold Spring Harbor protocols

PubMed ID

20889694