Preparation of early Drosophila embryonic fillets (before cuticle deposition)
Student Authors
Preethi RamachandranAcademic Program
NeuroscienceUMass Chan Affiliations
Graduate School of Biomedical Sciences, Neuroscience ProgramBudnik Lab
Neurobiology
Document Type
Journal ArticlePublication Date
2010-10-01Keywords
Animals; Cells, Cultured; Drosophila; Histological Techniques; Insect Proteins; OogoniaCell Biology
Developmental Biology
Laboratory and Basic Science Research
Neuroscience and Neurobiology
Metadata
Show full item recordAbstract
This article describes the dissection of early Drosophila embryos to generate flat or fillet preparations. For the procedure, a modified chamber or well is designed using glass slides and sealant. Embryos are dissected by removing the chorionic (outer) membrane (performed outside the chamber) and then removing the vitelline (inner) membrane (performed inside the chamber and under saline).Source
Cold Spring Harb Protoc. 2010 Oct 1;2010(10):pdb.prot5497. doi: 10.1101/pdb.prot5497. Link to article on publisher's site.DOI
10.1101/pdb.prot5497Permanent Link to this Item
http://hdl.handle.net/20.500.14038/33426PubMed ID
20889694Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1101/pdb.prot5497