Isolation and chromosomal localization of the human glutathione peroxidase gene
Biochemistry & Molecular Pharmacology
Graduate School of Biomedical Sciences; Department of Pediatrics
Medical Subject Headings
Base Sequence; Blotting, Southern; Chromosome Mapping; Chromosomes, Human; *Chromosomes, Human, Pair 3; Codon; DNA; Glutathione Peroxidase; Humans; Molecular Sequence Data; Nucleic Acid Hybridization; Sequence Homology, Nucleic Acid
Life Sciences | Medicine and Health Sciences
We have isolated cDNA clones for the gene, termed GPX1, encoding the major human selenoprotein, glutathione peroxidase. Sequence analysis confirmed previous findings that the unusual amino acid seleno-cysteine is encoded by the opal terminator codon UGA. Southern blot analysis of human genomic DNA with the GPX1 cDNA showed that restriction endonucleases without sites in the probe sequence produced three hybridizing bands at standard stringency, diminishing to one strongly and one weakly hybridizing band at high stringency. In situ hybridization localized the human GPX1 gene to a single site on chromosome 3, at region 3q11-13.1. Thus, three genomic sites bear sequence homology to the GPX1 cDNA, and the one most homologous maps to 3q11-13.1.
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Citation: Genomics. 1990 Feb;6(2):268-71.
Chada, Sunil; Le Beau, Michelle M.; Casey, Linda; and Newburger, Peter E., "Isolation and chromosomal localization of the human glutathione peroxidase gene" (1990). GSBS Student Publications. 194.