GSBS Student Publications

Student Author(s)

Kimberly S. Kerr; Yuly F. Fuentes-Medel; Ozge E. Tasdemir

GSBS Program

Neuroscience

UMMS Affiliation

Department of Neurobiology; Freeman Lab; Budnik Lab; Graduate School of Biomedical Sciences, Neuroscience Program

Date

2-19-2014

Document Type

Article

Medical Subject Headings

Animals; Chromatin Immunoprecipitation; Drosophila; Drosophila Proteins; Electrophysiological Processes; Homeodomain Proteins; Image Processing, Computer-Assisted; Immunohistochemistry; Microscopy, Confocal; Neuroglia; Neuromuscular Junction; RNA Interference; Real-Time Polymerase Chain Reaction; Receptors, Glutamate; Synapses; Transfection; Wnt Proteins

Disciplines

Developmental Neuroscience

Abstract

Glial cells are emerging as important regulators of synapse formation, maturation, and plasticity through the release of secreted signaling molecules. Here we use chromatin immunoprecipitation along with Drosophila genomic tiling arrays to define potential targets of the glial transcription factor Reversed polarity (Repo). Unexpectedly, we identified wingless (wg), a secreted morphogen that regulates synaptic growth at the Drosophila larval neuromuscular junction (NMJ), as a potential Repo target gene. We demonstrate that Repo regulates wg expression in vivo and that local glial cells secrete Wg at the NMJ to regulate glutamate receptor clustering and synaptic function. This work identifies Wg as a novel in vivo glial-secreted factor that specifically modulates assembly of the postsynaptic signaling machinery at the Drosophila NMJ.

Rights and Permissions

Citation: J Neurosci. 2014 Feb 19;34(8):2910-20. doi: 10.1523/JNEUROSCI.3714-13.2014. Link to article on publisher's site

DOI of Published Version

10.1523/JNEUROSCI.3714-13.2014

Comments

Publisher PDF posted as allowed by the publisher's author rights policy at http://www.jneurosci.org/site/misc/ifa_policies.xhtml#copyright.

Copyright of all material published in The Journal of Neuroscience remains with the authors. The authors grant the Society for Neuroscience an exclusive license to publish their work for the first 6 months. After 6 months the work becomes available to the public to copy, distribute, or display under a Creative Commons Attribution 4.0 International (CC BY 4.0) license.

Related Resources

Link to Article in PubMed

Keywords

Drosophila, NMJ, glia, synapse, wnt/Wg

Journal Title

The Journal of neuroscience : the official journal of the Society for Neuroscience

PubMed ID

24553932

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

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