GSBS Student Publications

Title

Distinct Notch signaling outputs pattern the developing arterial system

Student Author(s)

John C. Moore

GSBS Program

Interdisciplinary Graduate Program

UMMS Affiliation

Program in Gene Function and Expression

Date

4-1-2014

Document Type

Article

Medical Subject Headings

Animals; Animals, Genetically Modified; Arteries; Body Patterning; Cell Differentiation; Embryo, Nonmammalian; Endothelium, Vascular; Morphogenesis; Neovascularization, Physiologic; Receptors, Notch; Signal Transduction; Veins; Zebrafish

Disciplines

Cell and Developmental Biology | Developmental Biology

Abstract

Differentiation of arteries and veins is essential for the development of a functional circulatory system. In vertebrate embryos, genetic manipulation of Notch signaling has demonstrated the importance of this pathway in driving artery endothelial cell differentiation. However, when and where Notch activation occurs to affect endothelial cell fate is less clear. Using transgenic zebrafish bearing a Notch-responsive reporter, we demonstrate that Notch is activated in endothelial progenitors during vasculogenesis prior to blood vessel morphogenesis and is maintained in arterial endothelial cells throughout larval stages. Furthermore, we find that endothelial progenitors in which Notch is activated are committed to a dorsal aorta fate. Interestingly, some arterial endothelial cells subsequently downregulate Notch signaling and then contribute to veins during vascular remodeling. Lineage analysis, together with perturbation of both Notch receptor and ligand function, further suggests several distinct developmental windows in which Notch signaling acts to promote artery commitment and maintenance. Together, these findings demonstrate that Notch acts in distinct contexts to initiate and maintain artery identity during embryogenesis.

Rights and Permissions

Citation: Development. 2014 Apr;141(7):1544-52. doi: 10.1242/dev.099986. Epub 2014 Mar 5. Link to article on publisher's site

Related Resources

Link to Article in PubMed

Journal Title

Development (Cambridge, England)

PubMed ID

24598161