Construction and application of site-specific artificial nucleases for targeted gene editing
Student Authors
Ankit GuptaUMass Chan Affiliations
Program in Gene Function and ExpressionDepartment of Biochemistry and Molecular Pharmacology
Document Type
Book ChapterPublication Date
2014-01-01Keywords
Animals; Animals, Genetically Modified; Base Sequence; DNA Primers; Deoxyribonucleases; Escherichia coli; Gene Targeting; *INDEL Mutation; Molecular Sequence Data; Protein Engineering; Substrate Specificity; Zebrafish; Zinc FingersBiochemistry, Biophysics, and Structural Biology
Genetics and Genomics
Genomics
Molecular Biology
Metadata
Show full item recordAbstract
Artificial nucleases have developed into powerful tools for introducing precise genome modifications in a wide variety of species. In this chapter the authors provide detailed protocols for rapidly constructing zinc finger nucleases (ZFNs) and TALE nucleases (TALENs) and evaluating their activity for the targeted generation of InDels within the zebrafish genome.Source
Methods Mol Biol. 2014;1101:267-303. doi: 10.1007/978-1-62703-721-1_13. Link to article on publisher's siteDOI
10.1007/978-1-62703-721-1_13Permanent Link to this Item
http://hdl.handle.net/20.500.14038/33387PubMed ID
24233786Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1007/978-1-62703-721-1_13