GSBS Student Publications

Title

Construction and application of site-specific artificial nucleases for targeted gene editing

Student Author(s)

Ankit Gupta

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology; Program in Gene Function and Expression

Date

1-2014

Document Type

Book Chapter

Medical Subject Headings

Animals; Animals, Genetically Modified; Base Sequence; DNA Primers; Deoxyribonucleases; Escherichia coli; Gene Targeting; *INDEL Mutation; Molecular Sequence Data; Protein Engineering; Substrate Specificity; Zebrafish; Zinc Fingers

Disciplines

Biochemistry, Biophysics, and Structural Biology | Genetics and Genomics | Genomics | Molecular Biology

Abstract

Artificial nucleases have developed into powerful tools for introducing precise genome modifications in a wide variety of species. In this chapter the authors provide detailed protocols for rapidly constructing zinc finger nucleases (ZFNs) and TALE nucleases (TALENs) and evaluating their activity for the targeted generation of InDels within the zebrafish genome.

Rights and Permissions

Citation: Methods Mol Biol. 2014;1101:267-303. doi: 10.1007/978-1-62703-721-1_13. Link to article on publisher's site

Related Resources

Link to Article in PubMed

Journal Title

Methods in molecular biology (Clifton, N.J.)

PubMed ID

24233786