Construction and application of site-specific artificial nucleases for targeted gene editing
Biochemistry & Molecular Pharmacology
Department of Biochemistry and Molecular Pharmacology; Program in Gene Function and Expression
Medical Subject Headings
Animals; Animals, Genetically Modified; Base Sequence; DNA Primers; Deoxyribonucleases; Escherichia coli; Gene Targeting; *INDEL Mutation; Molecular Sequence Data; Protein Engineering; Substrate Specificity; Zebrafish; Zinc Fingers
Biochemistry, Biophysics, and Structural Biology | Genetics and Genomics | Genomics | Molecular Biology
Artificial nucleases have developed into powerful tools for introducing precise genome modifications in a wide variety of species. In this chapter the authors provide detailed protocols for rapidly constructing zinc finger nucleases (ZFNs) and TALE nucleases (TALENs) and evaluating their activity for the targeted generation of InDels within the zebrafish genome.
Rights and Permissions
Citation: Methods Mol Biol. 2014;1101:267-303. doi: 10.1007/978-1-62703-721-1_13. Link to article on publisher's site
Methods in molecular biology (Clifton, N.J.)
Kok, Fatma O.; Gupta, Ankit; Lawson, Nathan D.; and Wolfe, Scot A., "Construction and application of site-specific artificial nucleases for targeted gene editing" (2014). GSBS Student Publications. 1912.