GSBS Student Publications

Student Author(s)

Liang Meng Wee

GSBS Program

Interdisciplinary Graduate Program

UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology; RNA Therapeutics Institute

Date

4-1-2014

Document Type

Article

Medical Subject Headings

Animals; Conserved Sequence; *Evolution, Molecular; *Gene Expression Regulation; MicroRNAs; Nucleic Acid Conformation; Plants; RNA, Messenger; RNA, Small Interfering; Sea Anemones

Disciplines

Ecology and Evolutionary Biology | Genetics and Genomics | Genomics

Abstract

In bilaterians, which comprise most of extant animals, microRNAs (miRNAs) regulate the majority of messenger RNAs (mRNAs) via base-pairing of a short sequence (the miRNA "seed") to the target, subsequently promoting translational inhibition and transcript instability. In plants, many miRNAs guide endonucleolytic cleavage of highly complementary targets. Because little is known about miRNA function in nonbilaterian animals, we investigated the repertoire and biological activity of miRNAs in the sea anemone Nematostella vectensis, a representative of Cnidaria, the sister phylum of Bilateria. Our work uncovers scores of novel miRNAs in Nematostella, increasing the total miRNA gene count to 87. Yet only a handful are conserved in corals and hydras, suggesting that microRNA gene turnover in Cnidaria greatly exceeds that of other metazoan groups. We further show that Nematostella miRNAs frequently direct the cleavage of their mRNA targets via nearly perfect complementarity. This mode of action resembles that of small interfering RNAs (siRNAs) and plant miRNAs. It appears to be common in Cnidaria, as several of the miRNA target sites are conserved among distantly related anemone species, and we also detected miRNA-directed cleavage in Hydra. Unlike in bilaterians, Nematostella miRNAs are commonly coexpressed with their target transcripts. In light of these findings, we propose that post-transcriptional regulation by miRNAs functions differently in Cnidaria and Bilateria. The similar, siRNA-like mode of action of miRNAs in Cnidaria and plants suggests that this may be an ancestral state.

Rights and Permissions

Citation: Genome Res. 2014 Apr;24(4):651-63. doi: 10.1101/gr.162503.113. Epub 2014 Mar 18. Link to article on publisher's site

DOI of Published Version

10.1101/gr.162503.113

Comments

© 2014 Moran et al.; Published by Cold Spring Harbor Laboratory Press.

This article, published in Genome Research, is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.

Related Resources

Link to Article in PubMed

Journal Title

Genome research

PubMed ID

24642861

Creative Commons License

Creative Commons Attribution-Noncommercial 3.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial 3.0 License

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