GSBS Student Publications

Title

Noncoding RNA. piRNA-guided transposon cleavage initiates Zucchini-dependent, phased piRNA production

Student Author(s)

Bo W. Han; Wei Wang

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

RNA Therapeutics Institute; Department of Biochemistry and Molecular Pharmacology; Program in Bioinformatics and Integrative Biology

Date

5-15-2015

Document Type

Article

Medical Subject Headings

Animals; Argonaute Proteins; Drosophila Proteins; Drosophila melanogaster; Endoribonucleases; Female; Germ Cells; Male; Metabolic Networks and Pathways; Mice; Ovary; Peptide Initiation Factors; *RNA Cleavage; RNA, Guide; RNA, Small Interfering; *Retroelements; Testis

Disciplines

Biochemistry | Bioinformatics | Genetics and Genomics

Abstract

PIWI-interacting RNAs (piRNAs) protect the animal germ line by silencing transposons. Primary piRNAs, generated from transcripts of genomic transposon "junkyards" (piRNA clusters), are amplified by the "ping-pong" pathway, yielding secondary piRNAs. We report that secondary piRNAs, bound to the PIWI protein Ago3, can initiate primary piRNA production from cleaved transposon RNAs. The first ~26 nucleotides (nt) of each cleaved RNA becomes a secondary piRNA, but the subsequent ~26 nt become the first in a series of phased primary piRNAs that bind Piwi, allowing piRNAs to spread beyond the site of RNA cleavage. The ping-pong pathway increases only the abundance of piRNAs, whereas production of phased primary piRNAs from cleaved transposon RNAs adds sequence diversity to the piRNA pool, allowing adaptation to changes in transposon sequence.

Rights and Permissions

Citation: Science. 2015 May 15;348(6236):817-21. doi: 10.1126/science.aaa1264. Link to article on publisher's site

DOI of Published Version

10.1126/science.aaa1264

Related Resources

Link to Article in PubMed

Journal Title

Science (New York, N.Y.)

PubMed ID

25977554