Student Author(s)

Samriddha Ray; Sneha Gupta

GSBS Program

Interdisciplinary Graduate Program

UMMS Affiliation

Department of Molecular Genetics and Microbiology

Date

9-6-2010

Document Type

Article

Medical Subject Headings

Actins; Cell Division; Cytokinesis; Green Fluorescent Proteins; Interphase; *Mitosis; Recombinant Fusion Proteins; *Schizosaccharomyces; Schizosaccharomyces pombe Proteins; Signal Transduction

Disciplines

Cell Biology | Cellular and Molecular Physiology

Abstract

The mechanisms that regulate cytoskeletal remodeling during the transition between mitosis and interphase are poorly understood. In fission yeast the MOR pathway promotes actin polarization to cell tips in interphase, whereas the SIN signaling pathway drives actomyosin ring assembly and cytokinesis. We show that the SIN inhibits MOR signaling in mitosis by interfering with Nak1 kinase-mediated activation of the most downstream MOR component, the NDR family kinase Orb6. Inactivation of the MOR may be a key function of the SIN because attenuation of MOR signaling rescued the cytokinetic defects of SIN mutants and allowed weak SIN signaling to trigger ectopic cytokinesis. Furthermore, failure to inhibit the MOR is toxic when the cell division apparatus is compromised. Together, our results reveal a mutually antagonistic relationship between the SIN and MOR pathways, which is important for completion of cytokinesis and coordination of cytoskeletal remodeling at the mitosis-to-interphase transition.

Rights and Permissions

Citation: Ray S, Kume K, Gupta S, Ge W, alasubramanian M, Hirata D, McCollum D. The mitosis-to-interphase transition is coordinated by cross talk between the SIN and MOR pathways in Schizosaccharomyces pombe. J Cell Biol. 2010 Sep 6;190(5):793-805. doi: 10.1083/jcb.201002055. Link to article on publisher's site

Comments

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described athttp://creativecommons.org/licenses/by-nc-sa/3.0/).

Related Resources

Link to Article in PubMed