FBF represses the Cip/Kip cell-cycle inhibitor CKI-2 to promote self-renewal of germline stem cells in C. elegans
Biochemistry & Molecular Pharmacology
Department of Biochemistry and Molecular Pharmacology
Medical Subject Headings
Animals; Caenorhabditis elegans; Caenorhabditis elegans Proteins; *Cell Cycle; Cells, Cultured; Cyclin-Dependent Kinase Inhibitor Proteins; *Gene Expression Regulation; RNA-Binding Proteins; Stem Cells
Cell Biology | Genetics and Genomics | Molecular Genetics
Although the decision between stem cell self-renewal and differentiation has been linked to cell-cycle modifications, our understanding of cell-cycle regulation in stem cells is very limited. Here, we report that FBF/Pumilio, a conserved RNA-binding protein, promotes self-renewal of germline stem cells by repressing CKI-2(Cip/Kip), a Cyclin E/Cdk2 inhibitor. We have previously shown that repression of CYE-1 (Cyclin E) by another RNA-binding protein, GLD-1/Quaking, promotes germ cell differentiation. Together, these findings suggest that a post-transcriptional regulatory circuit involving FBF and GLD-1 controls the self-renewal versus differentiation decision in the germline by promoting high CYE-1/CDK-2 activity in stem cells, and inhibiting CYE-1/CDK-2 activity in differentiating cells.
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Citation: EMBO J. 2011 Aug 5;30(18):3823-9. doi: 10.1038/emboj.2011.263. Link to article on publisher's site
The EMBO journal
Kalchhauser, Irene; Farley, Brian M.; Pauli, Sandra; Ryder, Sean P.; and Ciosk, Rafal, "FBF represses the Cip/Kip cell-cycle inhibitor CKI-2 to promote self-renewal of germline stem cells in C. elegans" (2011). GSBS Student Publications. 1819.