Similarity of mouse perivascular and brown adipose tissues and their resistance to diet-induced inflammation
Authors
Fitzgibbons, Timothy P.Kogan, Sophia
Aouadi, Myriam
Hendricks, Gregory M.
Straubhaar, Juerg R.
Czech, Michael P.
Student Authors
Timothy FitzgibbonsUMass Chan Affiliations
Department of Cell BiologyDepartment of Cardiology
Program in Molecular Medicine
Document Type
Journal ArticlePublication Date
2011-07-19Keywords
Adipose Tissue; Adipose Tissue, Brown; Animals; Aorta, Thoracic; Apoptosis Regulatory Proteins; Blood Vessels; Diet; Dietary Fats; Flow Cytometry; Immunohistochemistry; Inflammation; Insulin; Ion Channels; Male; Mice; Mice, Inbred C57BL; Microarray Analysis; Microscopy, Electron, Transmission; Mitochondria, Heart; Mitochondrial Proteins; Neutrophil Infiltration; Reverse Transcriptase Polymerase Chain ReactionCardiovascular Diseases
Genetics and Genomics
Molecular Genetics
Nutritional and Metabolic Diseases
Metadata
Show full item recordAbstract
Thoracic perivascular adipose tissue (PVAT) is a unique adipose depot that likely influences vascular function and susceptibility to pathogenesis in obesity and the metabolic syndrome. Surprisingly, PVAT has been reported to share characteristics of both brown and white adipose, but a detailed direct comparison to interscapular brown adipose tissue (BAT) has not been performed. Here we show by full genome DNA microarray analysis that global gene expression profiles of PVAT are virtually identical to BAT, with equally high expression of Ucp-1, Cidea, and other genes known to be uniquely or very highly expressed in BAT. PVAT and BAT also displayed nearly identical phenotypes upon immunohistochemical analysis, and electron microscopy confirmed that PVAT contained multilocular lipid droplets and abundant mitochondria. Compared with white adipose tissue (WAT), PVAT and BAT from C57BL6/J mice fed a high-fat diet for 13 wk had markedly lower expression of immune cell-enriched mRNAs, suggesting resistance to obesity-induced inflammation. Indeed, staining of BAT and PVAT for macrophage markers (F4/80 and CD68) in obese mice showed virtually no macrophage infiltration, and FACS analysis of BAT confirmed the presence of very few CD11b(+)/CD11c(+) macrophages in BAT (1.0%) compared with WAT (31%). In summary, murine PVAT from the thoracic aorta is virtually identical to interscapular BAT, is resistant to diet-induced macrophage infiltration, and thus may play an important role in protecting the vascular bed from inflammatory stress.Source
10.1152/ajpheart.00376.2011. Epub 2011 Jul 15. Link to article on publisher's siteDOI
10.1152/ajpheart.00376.2011Permanent Link to this Item
http://hdl.handle.net/20.500.14038/33277PubMed ID
21765057Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1152/ajpheart.00376.2011