GSBS Student Publications

Student Author(s)

Nathan Moore

GSBS Program

Cancer Biology

UMMS Affiliation

Department of Medicine, Division of Gastroenterology; Department of Cancer Biology

Date

11-10-2012

Document Type

Article

Medical Subject Headings

Animals; Antineoplastic Combined Chemotherapy Protocols; Cell Line, Tumor; Cell Proliferation; *Drug Resistance, Neoplasm; Fluoresceins; Fluorescent Dyes; Fluorouracil; Humans; Mice; Mice, Inbred NOD; Mice, SCID; Neoplasms, Experimental; Organoplatinum Compounds; Succinimides; Xenograft Model Antitumor Assays

Disciplines

Cancer Biology | Oncology

Abstract

Tumor recurrence after chemotherapy is a major cause of patient morbidity and mortality. Recurrences are thought to be secondary to small subsets of cancer cells that are better able to survive traditional forms of chemotherapy and thus drive tumor regrowth. The ability to isolate and better characterize these therapy-resistant cells is critical for the future development of targeted therapies aimed at achieving more robust and long-lasting responses. Using a novel application for the proliferation marker carboxyfluorescein diacetate, succinimidyl ester (CFSE), we have identified a population of slow-cycling, label-retaining tumor cells in both in vitro sphere cultures and in vivo xenograft models. Strikingly, label-retaining cells exhibit a multifold increase in ability to survive traditional forms of chemotherapy and reenter the cell cycle. Further, we demonstrate the innovative application of CFSE to live sort slow-cycling tumor cells and validate their chemoresistance and tumorigenic potential.

Rights and Permissions

Citation: Nov 11. Link to article on publisher's site

DOI of Published Version

10.1089/scd.2011.0477

Related Resources

Link to Article in PubMed

Journal Title

Stem cells and development

PubMed ID

21973238

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