Title

dSarm/Sarm1 is required for activation of an injury-induced axon death pathway

Student Author(s)

Jeannette Osterloh; Timothy Rooney; Michelle Avery; Jennifer MacDonald; Jennifer Zeigenfuss

GSBS Program

Neuroscience

UMMS Affiliation

Department of Neurobiology; Department of Neurology

Date

7-27-2012

Document Type

Article

Medical Subject Headings

Animals; Animals, Genetically Modified; Apoptosis; Armadillo Domain Proteins; Axons; Axotomy; Cell Survival; Cells, Cultured; Cytoskeletal Proteins; Denervation; Drosophila; Drosophila Proteins; Mice; Mutation; Neurons; Sciatic Nerve; Signal Transduction; Superior Cervical Ganglion; Tissue Culture Techniques; *Wallerian Degeneration

Disciplines

Life Sciences | Medicine and Health Sciences | Neuroscience and Neurobiology

Abstract

Axonal and synaptic degeneration is a hallmark of peripheral neuropathy, brain injury, and neurodegenerative disease. Axonal degeneration has been proposed to be mediated by an active autodestruction program, akin to apoptotic cell death; however, loss-of-function mutations capable of potently blocking axon self-destruction have not been described. Here, we show that loss of the Drosophila Toll receptor adaptor dSarm (sterile alpha/Armadillo/Toll-Interleukin receptor homology domain protein) cell-autonomously suppresses Wallerian degeneration for weeks after axotomy. Severed mouse Sarm1 null axons exhibit remarkable long-term survival both in vivo and in vitro, indicating that Sarm1 prodegenerative signaling is conserved in mammals. Our results provide direct evidence that axons actively promote their own destruction after injury and identify dSarm/Sarm1 as a member of an ancient axon death signaling pathway.

Comments

Citation: Science. 2012 Jul 27;337(6093):481-4. Epub 2012 Jun 7. Link to article on publisher's site

Related Resources

Link to Article in PubMed