CD8 T cell cross-reactivity networks mediate heterologous immunity in human EBV and murine vaccinia virus infections
Immunology & Virology Program
Department of Pathology
Medical Subject Headings
Adolescent; Adoptive Transfer; Animals; CD8-Positive T-Lymphocytes; Cell Line, Tumor; Cells, Cultured; Coculture Techniques; Epitopes, T-Lymphocyte; Epstein-Barr Virus Infections; Humans; Immunity, Cellular; Immunologic Memory; Influenza, Human; Lymphocyte Activation; Lymphocytic Choriomeningitis; Male; Mice; Mice, Congenic; Mice, Inbred C57BL; Vaccinia; Young Adult
Immunology and Infectious Disease | Life Sciences | Medicine and Health Sciences
In this study, we demonstrate complex networks of CD8 T cell cross-reactivities between influenza A virus and EBV in humans and between lymphocytic choriomeningitis virus and vaccinia virus in mice. We also show directly that cross-reactive T cells mediate protective heterologous immunity in mice. Subsets of T cell populations reactive with one epitope cross-reacted with either of several other epitopes encoded by the same or the heterologous virus. Human T cells specific to EBV-encoded BMLF1(280-288) could be cross-reactive with two influenza A virus or two other EBV epitopes. Mouse T cells specific to the vaccinia virus-encoded a11r(198-205) could be cross-reactive with three different lymphocytic choriomeningitis virus, one Pichinde virus, or one other vaccinia virus epitope. Patterns of cross-reactivity differed among individuals, reflecting the private specificities of the host's immune repertoire and divergence in the abilities of T cell populations to mediate protective immunity. Defining such cross-reactive networks between commonly encountered human pathogens may facilitate the design of vaccines.
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Citation: J Immunol. 2010 Mar 15;184(6):2825-38. Epub 2010 Feb 17.