Yeast one-hybrid assays for gene-centered human gene regulatory network mapping
Interdisciplinary Graduate Program
Program in Gene Function and Expression; Program in Molecular Medicine; Program in Systems Biology; Department of Biochemistry and Molecular Pharmacology
Medical Subject Headings
Two-Hybrid System Techniques; High-Throughput Screening Assays; Gene Regulatory Networks; Protein Interaction Mapping; Transcription Factors
Biochemistry, Biophysics, and Structural Biology | Genetics and Genomics | Life Sciences | Medicine and Health Sciences
Gateway-compatible yeast one-hybrid (Y1H) assays provide a convenient gene-centered (DNA to protein) approach to identify transcription factors that can bind a DNA sequence of interest. We present Y1H resources, including clones for 988 of 1,434 (69%) predicted human transcription factors, that can be used to detect both known and new interactions between human DNA regions and transcription factors.
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Citation: Nat Methods. 2011 Oct 30;8(12):1050-2. doi: 10.1038/nmeth.1764. Link to article on publisher's site
Reece-Hoyes, John S.; Barutcu, A. Rasim; McCord, Rachel Patton; Jeong, Jun Seop; Jiang, Lizhi; Macwilliams, Andrew; Yang, Xinping; Salehi-Ashtiani, Kourosh; Hill, David E.; Blackshaw, Seth; Zhu, Heng; Dekker, Job; and Walhout, Albertha J. M., "Yeast one-hybrid assays for gene-centered human gene regulatory network mapping" (2011). GSBS Student Publications. 1763.