Title

Yeast one-hybrid assays for gene-centered human gene regulatory network mapping

Student Author(s)

Ahmet Rasim Barutcu

GSBS Program

Interdisciplinary Graduate Program

UMMS Affiliation

Program in Gene Function and Expression; Program in Molecular Medicine; Program in Systems Biology; Department of Biochemistry and Molecular Pharmacology

Date

10-30-2011

Document Type

Article

Medical Subject Headings

Two-Hybrid System Techniques; High-Throughput Screening Assays; Gene Regulatory Networks; Protein Interaction Mapping; Transcription Factors

Disciplines

Biochemistry, Biophysics, and Structural Biology | Genetics and Genomics | Life Sciences | Medicine and Health Sciences

Abstract

Gateway-compatible yeast one-hybrid (Y1H) assays provide a convenient gene-centered (DNA to protein) approach to identify transcription factors that can bind a DNA sequence of interest. We present Y1H resources, including clones for 988 of 1,434 (69%) predicted human transcription factors, that can be used to detect both known and new interactions between human DNA regions and transcription factors.

Rights and Permissions

Citation: Nat Methods. 2011 Oct 30;8(12):1050-2. doi: 10.1038/nmeth.1764. Link to article on publisher's site

Related Resources

Link to Article in PubMed