Graduate School of Biomedical Sciences; Program in Molecular Medicine; Department of Biochemistry and Molecular Pharmacology
Medical Subject Headings
Adenosine Triphosphatases; Bacterial Proteins; Base Pair Mismatch; Cisplatin; Conjugation, Genetic; Crosses, Genetic; DNA Helicases; *DNA Repair; DNA-Binding Proteins; Escherichia coli; Escherichia coli Proteins; MutS DNA Mismatch-Binding Protein; *Mutation; Oligonucleotides; *Recombination, Genetic
Life Sciences | Medicine and Health Sciences
DNA mismatch repair in Escherichia coli has been shown to be involved in two distinct processes: mutation avoidance, which removes potential mutations arising as replication errors, and antirecombination which prevents recombination between related, but not identical (homeologous), DNA sequences. We show that cells with the mutSDelta800 mutation (which removes the C-terminal 53 amino acids of MutS) on a multicopy plasmid are proficient for mutation avoidance. In interspecies genetic crosses, however, recipients with the mutSDelta800 mutation show increased recombination by up to 280-fold relative to mutS+. The MutSDelta800 protein binds to O6-methylguanine mismatches but not to intrastrand platinated GG cross-links, explaining why dam bacteria with the mutSDelta800 mutation are resistant to cisplatin, but not MNNG, toxicity. The results indicate that the C-terminal end of MutS is necessary for antirecombination and cisplatin sensitization, but less significant for mutation avoidance. The inability of MutSDelta800 to form tetramers may indicate that these are the active form of MutS.
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Citation: Nucleic Acids Res. 2005 Feb 24;33(4):1193-200. Print 2005. Link to article on publisher's site