GSBS Student Publications

Title

The MutS C terminus is essential for mismatch repair activity in vivo

GSBS Program

Biochemistry & Molecular Pharmacology

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Biochemistry and Molecular Pharmacology; Program in Molecular Medicine

Date

9-15-2005

Document Type

Article

Medical Subject Headings

Adenosine Triphosphatases; Bacterial Proteins; Base Pair Mismatch; DNA Repair; DNA-Binding Proteins; Escherichia coli; *Escherichia coli Proteins; MutS DNA Mismatch-Binding Protein; *Mutation

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

An Escherichia coli K-12 strain was constructed with a chromosomal deletion (mutSdelta800) in the mutS gene that produced the removal of the C-terminal 53 amino acids which are not present in the MutS crystal structure. This strain has a MutS null phenotype for mutation avoidance, anti-recombination, and sensitivity to cytotoxic agents in a dam mutant background.

Rights and Permissions

Citation: J Bacteriol. 2005 Sep;187(18):6577-9. Link to article on publisher's site

Related Resources

Link to article in PubMed

Journal Title

Journal of bacteriology

PubMed ID

16159793