Title
The MutS C terminus is essential for mismatch repair activity in vivo
GSBS Program
Biochemistry & Molecular Pharmacology
UMMS Affiliation
Graduate School of Biomedical Sciences; Department of Biochemistry and Molecular Pharmacology; Program in Molecular Medicine
Date
9-15-2005
Document Type
Article
Medical Subject Headings
Adenosine Triphosphatases; Bacterial Proteins; Base Pair Mismatch; DNA Repair; DNA-Binding Proteins; Escherichia coli; *Escherichia coli Proteins; MutS DNA Mismatch-Binding Protein; *Mutation
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
An Escherichia coli K-12 strain was constructed with a chromosomal deletion (mutSdelta800) in the mutS gene that produced the removal of the C-terminal 53 amino acids which are not present in the MutS crystal structure. This strain has a MutS null phenotype for mutation avoidance, anti-recombination, and sensitivity to cytotoxic agents in a dam mutant background.
Rights and Permissions
Citation: J Bacteriol. 2005 Sep;187(18):6577-9. Link to article on publisher's site