GSBS Student Publications

Title

Preparation of late Drosophila embryonic fillets

Student Author(s)

Preethi Ramachandran

GSBS Program

Neuroscience

UMMS Affiliation

Department of Neurobiology; Budnik Lab; Graduate School of Biomedical Sciences, Neuroscience Program

Date

10-5-2010

Document Type

Article

Medical Subject Headings

Animals; Cell Culture Techniques; Drosophila; Histological Techniques; Oogonia

Disciplines

Neuroscience and Neurobiology

Abstract

Beyond the age of ~15 h (at 25 degrees C), Drosophila embryos become impenetrable to aqueous fixatives and staining methods as a result of cuticle deposition. This problem can be overcome using flat or fillet preparations, as described in this protocol.

Rights and Permissions

Citation: Cold Spring Harb Protoc. 2010 Oct 1;2010(10):pdb.prot5498. doi: 10.1101/pdb.prot5498.

DOI of Published Version

10.1101/pdb.prot5498

Related Resources

Link to Article in PubMed

Journal Title

Cold Spring Harbor protocols

PubMed ID

20889695