Title
Preparation of late Drosophila embryonic fillets
GSBS Program
Neuroscience
UMMS Affiliation
Department of Neurobiology
Date
10-5-2010
Document Type
Article
Medical Subject Headings
Animals; Cell Culture Techniques; Drosophila; Histological Techniques; Oogonia
Disciplines
Life Sciences | Medicine and Health Sciences | Neuroscience and Neurobiology
Abstract
Beyond the age of ~15 h (at 25 degrees C), Drosophila embryos become impenetrable to aqueous fixatives and staining methods as a result of cuticle deposition. This problem can be overcome using flat or fillet preparations, as described in this protocol.
Rights and Permissions
Citation: Cold Spring Harb Protoc. 2010 Oct 1;2010(10):pdb.prot5498. doi: 10.1101/pdb.prot5498.
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