Title
Preparation of early Drosophila embryonic fillets (before cuticle deposition)
GSBS Program
Neuroscience
UMMS Affiliation
Department of Neurobiology
Date
10-5-2010
Document Type
Article
Medical Subject Headings
Animals; Cells, Cultured; Drosophila; Histological Techniques; Insect Proteins; Oogonia
Disciplines
Life Sciences | Medicine and Health Sciences | Neuroscience and Neurobiology
Abstract
This article describes the dissection of early Drosophila embryos to generate flat or fillet preparations. For the procedure, a modified chamber or well is designed using glass slides and sealant. Embryos are dissected by removing the chorionic (outer) membrane (performed outside the chamber) and then removing the vitelline (inner) membrane (performed inside the chamber and under saline).
Rights and Permissions
Citation: Cold Spring Harb Protoc. 2010 Oct 1;2010(10):pdb.prot5497. doi: 10.1101/pdb.prot5497.