Electron microscopy of Drosophila larval neuromuscular junctions
Department of Neurobiology
Medical Subject Headings
Animals; Dissection; Drosophila; Larva; Microscopy, Electron; Muscles; Neuromuscular Junction
Life Sciences | Medicine and Health Sciences | Neuroscience and Neurobiology
Over the last two decades, the Drosophila larval neuromuscular junction has gained immense popularity as a model system for the study of synaptic development, function, and plasticity. With this model, it is easy to visualize synapses and manipulate the system genetically with a high degree of temporal and spatial control, which makes it ideal for resolving problems in synaptic physiology and development. This article describes the preparation of Drosophila larval body-wall muscles for imaging by electron microscopy. The samples are fixed, stained, and embedded in Spurr’s resin before being sectioned for electron microscopy.
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Citation: Cold Spring Harb Protoc. 2010 Aug 1;2010(8):pdb.prot5474. doi: 10.1101/pdb.prot5474.