GSBS Student Publications

Title

Electron microscopy of Drosophila larval neuromuscular junctions

Student Author(s)

Preethi Ramachandran

GSBS Program

Neuroscience

UMMS Affiliation

Department of Neurobiology; Budnik Lab; Graduate School of Biomedical Sciences, Neuroscience Program

Date

8-4-2010

Document Type

Article

Medical Subject Headings

Animals; Dissection; Drosophila; Larva; Microscopy, Electron; Muscles; Neuromuscular Junction

Disciplines

Neuroscience and Neurobiology

Abstract

Over the last two decades, the Drosophila larval neuromuscularjunction has gained immense popularity as a model system forthe study of synaptic development, function, and plasticity.With this model, it is easy to visualize synapses and manipulatethe system genetically with a high degree of temporal and spatialcontrol, which makes it ideal for resolving problems in synapticphysiology and development. This article describes the preparationof Drosophila larval body-wall muscles for imaging by electronmicroscopy. The samples are fixed, stained, and embedded inSpurr’s resin before being sectioned for electron microscopy.

Rights and Permissions

Citation: Cold Spring Harb Protoc. 2010 Aug 1;2010(8):pdb.prot5474. doi: 10.1101/pdb.prot5474.

DOI of Published Version

10.1101/pdb.prot5474

Related Resources

Link to Article in PubMed

Journal Title

Cold Spring Harbor protocols

PubMed ID

20679383