Title
Activity paradigm to induce Drosophila larval neuromuscular junction growth
GSBS Program
Neuroscience
UMMS Affiliation
Department of Neurobiology
Date
8-4-2010
Document Type
Article
Medical Subject Headings
Animals; Dissection; Drosophila melanogaster; Electrophysiology; Imaging, Three-Dimensional; Larva; Motor Activity; Neuromuscular Junction; Tissue Fixation
Disciplines
Life Sciences | Medicine and Health Sciences | Neuroscience and Neurobiology
Abstract
This article describes a method for spaced stimulation of Drosophila motor neurons. The neurons can be stimulated for short periods of time (~2 h) and then fixed (or imaged live). The method allows one to examine the immediate effects of altering activity on synaptic protein localization as well as neuromuscular junction structure and function.
Rights and Permissions
Citation: Cold Spring Harb Protoc. 2010 Aug 1;2010(8):pdb.prot5473. doi: 10.1101/pdb.prot5473.