Activity paradigm to induce Drosophila larval neuromuscular junction growth
Department of Neurobiology
Medical Subject Headings
Animals; Dissection; Drosophila melanogaster; Electrophysiology; Imaging, Three-Dimensional; Larva; Motor Activity; Neuromuscular Junction; Tissue Fixation
Life Sciences | Medicine and Health Sciences | Neuroscience and Neurobiology
This article describes a method for spaced stimulation of Drosophila motor neurons. The neurons can be stimulated for short periods of time (~2 h) and then fixed (or imaged live). The method allows one to examine the immediate effects of altering activity on synaptic protein localization as well as neuromuscular junction structure and function.
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Citation: Cold Spring Harb Protoc. 2010 Aug 1;2010(8):pdb.prot5473. doi: 10.1101/pdb.prot5473.