GSBS Student Publications

Title

Activity paradigm to induce Drosophila larval neuromuscular junction growth

Student Author(s)

Preethi Ramachandran

GSBS Program

Neuroscience

UMMS Affiliation

Department of Neurobiology; Budnik Lab; Graduate School of Biomedical Sciences, Neuroscience Program

Date

8-4-2010

Document Type

Article

Medical Subject Headings

Animals; Dissection; Drosophila melanogaster; Electrophysiology; Imaging, Three-Dimensional; Larva; Motor Activity; Neuromuscular Junction; Tissue Fixation

Disciplines

Neuroscience and Neurobiology

Abstract

This article describes a method for spaced stimulation of Drosophilamotor neurons. The neurons can be stimulated for short periodsof time (~2 h) and then fixed (or imaged live). The method allowsone to examine the immediate effects of altering activity onsynaptic protein localization as well as neuromuscular junctionstructure and function.

Rights and Permissions

Citation: Cold Spring Harb Protoc. 2010 Aug 1;2010(8):pdb.prot5473. doi: 10.1101/pdb.prot5473.

DOI of Published Version

10.1101/pdb.prot5473

Related Resources

Link to Article in PubMed

Journal Title

Cold Spring Harbor protocols

PubMed ID

20679382