Student Authors
Preethi RamachandranAcademic Program
NeuroscienceUMass Chan Affiliations
Graduate School of Biomedical Sciences, Neuroscience ProgramBudnik Lab
Neurobiology
Document Type
Journal ArticlePublication Date
2010-08-04Keywords
Animals; Biological Assay; Drosophila melanogaster; *Endocytosis; Fluorescein-5-isothiocyanate; Larva; Protein TransportNeuroscience and Neurobiology
Metadata
Show full item recordAbstract
Over the last two decades, the Drosophila larval neuromuscularjunction has gained immense popularity as a model system forthe study of synaptic development, function, and plasticity.With this model, it is easy to visualize synapses and manipulatethe system genetically with a high degree of temporal and spatialcontrol, which makes it ideal for resolving problems in synapticphysiology and development. This protocol describes an assaythat can be used for observing the cycling of transmembraneproteins at the plasma membrane and their trafficking withincells when there are antibodies available that bind cell-surfaceepitopes in vivo.Source
Cold Spring Harb Protoc. 2010 Aug 1;2010(8):pdb.prot5472. doi: 10.1101/pdb.prot5472.DOI
10.1101/pdb.prot5472Permanent Link to this Item
http://hdl.handle.net/20.500.14038/33162PubMed ID
20679381Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1101/pdb.prot5472