GSBS Student Publications

Title

Internalization and trafficking assay for Drosophila larvae

Student Author(s)

Preethi Ramachandran

GSBS Program

Neuroscience

UMMS Affiliation

Department of Neurobiology; Budnik Lab; Graduate School of Biomedical Sciences, Neuroscience Program

Date

8-4-2010

Document Type

Article

Medical Subject Headings

Animals; Biological Assay; Drosophila melanogaster; *Endocytosis; Fluorescein-5-isothiocyanate; Larva; Protein Transport

Disciplines

Neuroscience and Neurobiology

Abstract

Over the last two decades, the Drosophila larval neuromuscularjunction has gained immense popularity as a model system forthe study of synaptic development, function, and plasticity.With this model, it is easy to visualize synapses and manipulatethe system genetically with a high degree of temporal and spatialcontrol, which makes it ideal for resolving problems in synapticphysiology and development. This protocol describes an assaythat can be used for observing the cycling of transmembraneproteins at the plasma membrane and their trafficking withincells when there are antibodies available that bind cell-surfaceepitopes in vivo.

Rights and Permissions

Citation: Cold Spring Harb Protoc. 2010 Aug 1;2010(8):pdb.prot5472. doi: 10.1101/pdb.prot5472.

DOI of Published Version

10.1101/pdb.prot5472

Related Resources

Link to Article in PubMed

Journal Title

Cold Spring Harbor protocols

PubMed ID

20679381