Fm1-43 labeling of Drosophila larval neuromuscular junctions
Department of Neurobiology; Budnik Lab; Graduate School of Biomedical Sciences, Neuroscience Program
Medical Subject Headings
Animals; Dissection; Drosophila melanogaster; Larva; Neuromuscular Junction; Pyridinium Compounds; Quaternary Ammonium Compounds; Staining and Labeling
Neuroscience and Neurobiology
Over the last two decades, the Drosophila larval neuromuscularjunction (NMJ) has gained immense popularity as a model systemfor the study of synaptic development, function, and plasticity.With this model, it is easy to visualize synapses and manipulatethe system genetically with a high degree of temporal and spatialcontrol, which makes it ideal for resolving problems in synapticphysiology and development. Here, we describe a simple methodfor looking at vesicle recycling at the NMJ by labeling withFM1-43 dye.
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Citation: Cold Spring Harb Protoc. 2010 Aug 1;2010(8):pdb.prot5471. doi: 10.1101/pdb.prot5471.
Ramachandran, Preethi and Budnik, Vivian, "Fm1-43 labeling of Drosophila larval neuromuscular junctions" (2010). GSBS Student Publications. 1700.