Fm1-43 labeling of Drosophila larval neuromuscular junctions
Department of Neurobiology
Medical Subject Headings
Animals; Dissection; Drosophila melanogaster; Larva; Neuromuscular Junction; Pyridinium Compounds; Quaternary Ammonium Compounds; Staining and Labeling
Life Sciences | Medicine and Health Sciences | Neuroscience and Neurobiology
Over the last two decades, the Drosophila larval neuromuscular junction (NMJ) has gained immense popularity as a model system for the study of synaptic development, function, and plasticity. With this model, it is easy to visualize synapses and manipulate the system genetically with a high degree of temporal and spatial control, which makes it ideal for resolving problems in synaptic physiology and development. Here, we describe a simple method for looking at vesicle recycling at the NMJ by labeling with FM1-43 dye.
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Citation: Cold Spring Harb Protoc. 2010 Aug 1;2010(8):pdb.prot5471. doi: 10.1101/pdb.prot5471.