Title

Fm1-43 labeling of Drosophila larval neuromuscular junctions

Student Author(s)

Preethi Ramachandran

GSBS Program

Neuroscience

UMMS Affiliation

Department of Neurobiology

Date

8-4-2010

Document Type

Article

Medical Subject Headings

Animals; Dissection; Drosophila melanogaster; Larva; Neuromuscular Junction; Pyridinium Compounds; Quaternary Ammonium Compounds; Staining and Labeling

Disciplines

Life Sciences | Medicine and Health Sciences | Neuroscience and Neurobiology

Abstract

Over the last two decades, the Drosophila larval neuromuscular junction (NMJ) has gained immense popularity as a model system for the study of synaptic development, function, and plasticity. With this model, it is easy to visualize synapses and manipulate the system genetically with a high degree of temporal and spatial control, which makes it ideal for resolving problems in synaptic physiology and development. Here, we describe a simple method for looking at vesicle recycling at the NMJ by labeling with FM1-43 dye.

Rights and Permissions

Citation: Cold Spring Harb Protoc. 2010 Aug 1;2010(8):pdb.prot5471. doi: 10.1101/pdb.prot5471.

Related Resources

Link to Article in PubMed